Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing anisotropic hydrogel

An anisotropic and hydrogel technology, applied in the field of 3D printing and biomaterials, can solve problems that do not conform to the structural composition characteristics of organisms, achieve high precision and simplify the process

Active Publication Date: 2021-04-27
INST OF ZOOLOGY CHINESE ACAD OF SCI
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing anisotropic hydrogels are all prepared from different types of hydrogels, and the strategies for generating anisotropy are simple and do not conform to the highly complex structure and composition characteristics of living organisms.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing anisotropic hydrogel
  • Method for preparing anisotropic hydrogel
  • Method for preparing anisotropic hydrogel

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0085] Dissolve pigskin gelatin (G1890) in phosphate buffer solution to prepare gelatin solutions with a concentration of 80g / L, 100g / L, 120g / L and 140g / L, and use a rheometer to test its temperature change curve to determine the printing temperature.

[0086] Figure 4 The storage modulus and loss modulus of 80g / L, 100g / L, 120g / L and 140g / L gelatin solutions are shown as a function of temperature. From the intersection of the storage modulus curve and the loss modulus curve, the gel temperatures of 80g / L, 100g / L, 120g / L and 140g / L hydrogels were 24°C, 26°C, 26.5°C and 28°C respectively. °C to determine the printing temperature of each gelatin solution.

[0087] Using commercially available PLA filaments that can be used for 3D printing as raw materials, a 3D printer was used to print the mold. The specifications of the PLA wire are as follows: diameter 2.85±0.10mm, maximum roundness deviation 0.10mm, wire net weight 350g / 750g, wire length ~44m / ~95m.

Embodiment 1

[0089] Add the gelatin solution with a concentration of 80g / L into the barrel of the 3D printer. The temperature of the 3D printer platform is controlled at 10°C, the temperature of the nozzle is controlled at 24°C, the printing pressure is set to 0.12MPa, and the printing speed is set to 30mm / s. Default patterns such as image 3 As shown in A, the wire spacing is set to 1mm, and the number of printing layers is set to 1 layer. After the printed 80g / L gelatin solution is solidified, manually add 4.5mL of 100g / L gelatin solution into the mold, and add 15mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide 600 μL of aqueous solution of N-hydroxysuccinimide and 600 μL of 6 mM N-hydroxysuccinimide aqueous solution were cross-linked at 4 ° C for one hour in the dark, and then washed with phosphate buffer for 3 times for 15 min each time to obtain a hydrogel glue.

[0090] The mechanical properties of the hydrogel obtained in this embodiment were tested using a tensile tester. The hy...

Embodiment 2

[0092] Add the gelatin solution with a concentration of 80g / L into the barrel of the 3D printer. The temperature of the 3D printer platform is controlled at 10°C, the temperature of the nozzle is controlled at 24°C, the printing pressure is set to 0.12MPa, and the printing speed is set to 30mm / s. Default patterns such as image 3 As shown in B, the wire spacing is set to 1mm, the period is set to 2mm, the amplitude is set to 1mm, and the number of printing layers is set to 1 layer. After the printed 80g / L gelatin solution is solidified, manually add 4.5mL of 100g / L gelatin solution into the mold, and add 15mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide 600 μL of aqueous solution of N-hydroxysuccinimide and 600 μL of 6 mM N-hydroxysuccinimide aqueous solution were cross-linked at 4 ° C for one hour in the dark, and the cross-linking agent was washed away with phosphate buffer, 15 min / time, and washed 3 times in total to obtain a hydrogel .

[0093] The mechanical properties...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Fracture stressaaaaaaaaaa
Young's modulusaaaaaaaaaa
Fracture stressaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of 3D printing and biological materials, in particular to a method for preparing anisotropic hydrogel, the hydrogel prepared through the method and an application of the hydrogel. The method provided by the invention can be used for preparing the anisotropic hydrogel based on the same material, and has the advantages of higher precision and simplified process.

Description

technical field [0001] The invention relates to the fields of 3D printing and biomaterials, in particular to a method for preparing anisotropic hydrogel, the hydrogel prepared by the method, and the application of the hydrogel. Background technique [0002] Hydrogel is a gel with water as the dispersion medium. It is a polymer network system with soft properties, can maintain a certain shape, and can absorb a large amount of water. All water-soluble or hydrophilic polymers can form hydrogels through certain chemical cross-linking or physical cross-linking. Hydrogels formed from biocompatible polymers can be used in cell culture, tissue engineering, drug release and other fields. [0003] Biological tissues (such as skin, muscle, etc.) are anisotropic, and tissues in different parts of the living body usually have different anisotropy. Hydrogels used for tissue transplantation materials need to meet the anisotropy requirements of biological tissues in order to facilitate ti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J3/075C08J3/24C08L89/00B29C64/106B29C64/379B33Y10/00B33Y40/20B33Y70/00
CPCC08J3/075C08J3/24B29C64/106B29C64/379B33Y10/00B33Y40/00B33Y70/00C08J2389/00
Inventor 顾振高子力顾奇
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com