Gradient biomimetic mineralized collagen hydrogel and preparation method thereof
A collagen hydrogel and biomimetic mineralization technology, applied in the field of biomimetic materials, can solve problems affecting the repair and regeneration of soft and hard composite tissues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0038] The invention provides a preparation method of gradient biomimetic mineralized collagen hydrogel, comprising the following steps:
[0039] (1) gelling the type I collagen acid solution to obtain the first collagen hydrogel;
[0040] (2) mixing the first collagen hydrogel obtained in the step (1) with a mineralization solution for mineralization to obtain the first layer of mineralized collagen hydrogel;
[0041] (3) The first layer of mineralized collagen hydrogel obtained in the step (2) is placed at the bottom of the container, and then type I collagen acid solution is added to the container to form a gel to obtain two layers of collagen hydrogel ;
[0042] (4) mixing the two-layer collagen hydrogel obtained in the step (3) with the mineralization solution for mineralization to obtain two-layer mineralized collagen hydrogel;
[0043] (5) Place the two layers of mineralized collagen hydrogel obtained in step (4) at the bottom of the container, then add type I collage...
Embodiment 1
[0082] Extraction of type I collagen:
[0083] Before operation, perform high-pressure steam sterilization on all instruments, reagents, and containers;
[0084] Sampling: Take 50g of pig’s hoof tendon, soak it in double-resistant water for 15 minutes, cut it into minced meat with scissors, dissolve it in 500mL Tris solution, and stay overnight at 4°C; wash it 5 times with distilled water, filter it with gauze, dilute to 1.5L, add 45mL acetic acid, 5g Pepsin, placed at 4°C, stirred for 3 days;
[0085] Centrifugation and filtration: Dispense into 50mL centrifuge tubes, centrifuge at 4000rpm for 25 minutes at 4°C, recover collagen, dilute with 0.5M acetic acid, and filter again with gauze;
[0086] Salting out: 0.9M sodium chloride solution salting out, overnight at 4°C, stirring continuously during the period;
[0087] Re-centrifugation: Dispense into 50mL centrifuge tubes, centrifuge at 4000rpm at 4°C for 25 minutes, dissolve in 0.5M acetic acid overnight at 4°C;
[0088] ...
Embodiment 2
[0104] The gradient biomimetic mineralized collagen hydrogel prepared in Example 1 was separated to obtain the uppermost hydrogel, which was chopped and immersed in 0.1M ammonium bicarbonate (4°C, pH=7.8). The chopped hydrogel was homogenized until there were no visible fragments, then 2 μL of the homogenate was dropped onto a nickel mesh, air-dried, and stained with uranyl acetate for 10 seconds before TEM imaging, and finally Hitachi H-1010 (120kV ) observation by transmission electron microscope, the results are as follows Figure 8 shown.
[0105] From Figure 8 It can be seen that the collagen fibrils (the uppermost hydrogel) formed by self-assembly in Example 1 of the present invention have a periodic structure of 67 nm, and the diameter of the collagen fibrils is 60.6 ± 12.0 nm (N = 50), Similar to natural collagen fibrils, it shows that the uppermost hydrogel of the gradient biomimetic mineralized collagen hydrogel prepared by the present invention has the structural...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More