Device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and method and application thereof
A technology of Raman spectroscopy detection and Raman spectroscopy, which is applied in the field of fermentation broth detection, can solve the problems of cumbersome measurement steps, troublesome pre-processing, and hysteresis of detection results.
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Embodiment 1
[0058] Such as figure 1 As shown, a device for detecting polysaccharides in fermentation broth based on Raman spectroscopy, including a fermenter (1), a mirror (2), an optical fiber probe (3), optical fibers (4, 5), an excitation light source (6), a detection device (7), signal acquisition module (8), A / D conversion module (9), single-chip microcomputer (10), liquid crystal display screen (11), mobile phone (12) or computer (13); wherein, the reflecting mirror (2) Located in the fermenter (1), the excitation light source (6) is connected to the optical fiber probe (3) through the optical fiber (4), and the optical fiber probe (3) is further connected to the detector (7) through the optical fiber (5), and the detector (7) Further be connected with signal acquisition module (8), then signal acquisition module (8) is connected with A / D conversion module (9), A / D conversion module (9) is connected with further single-chip microcomputer (10), single-chip microcomputer (10) ) is co...
Embodiment 2
[0061] A device for detecting polysaccharides in fermentation broth based on Raman spectroscopy, the detection process is as follows:
[0062] (a) The incident light emitted by the exciting light source (6) enters the fermentation broth in the fermenter (1) through the optical fiber (4) and the optical fiber probe (3);
[0063] (b) The incident light is reflected by the mirror substrate, and the reflected light enters the detector (7) through the optical fiber probe (3) and the optical fiber (5);
[0064] (c) the detector (7) sends the detected Raman spectrum signal to the signal acquisition module (8);
[0065] (d) The signal collected by the signal acquisition module (8) is converted into a digital signal through the A / D conversion module (9);
[0066] (e) digital signal is transmitted to single-chip microcomputer (10);
[0067] (f) the single-chip microcomputer (10) carries out algorithmic processing to the digital signal, separates and calculates the spectrum of pure pol...
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