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Device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and method and application thereof

A technology of Raman spectroscopy detection and Raman spectroscopy, which is applied in the field of fermentation broth detection, can solve the problems of cumbersome measurement steps, troublesome pre-processing, and hysteresis of detection results.

Pending Publication Date: 2021-04-30
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The precision of the HPLC detection device is high, but this method takes too long and the pretreatment is troublesome; the minimum detection limit of the ELISA detection method is low and the sensitivity is high, but the detection steps of this method are cumbersome and require multiple washings; The determination steps of dinitrosalicylic acid method and phenol-sulfuric acid method are very cumbersome
These traditional determination methods cannot reflect the components in the fermentation broth in real time, and the detection results have serious hysteresis

Method used

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  • Device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and method and application thereof

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Embodiment 1

[0058] Such as figure 1 As shown, a device for detecting polysaccharides in fermentation broth based on Raman spectroscopy, including a fermenter (1), a mirror (2), an optical fiber probe (3), optical fibers (4, 5), an excitation light source (6), a detection device (7), signal acquisition module (8), A / D conversion module (9), single-chip microcomputer (10), liquid crystal display screen (11), mobile phone (12) or computer (13); wherein, the reflecting mirror (2) Located in the fermenter (1), the excitation light source (6) is connected to the optical fiber probe (3) through the optical fiber (4), and the optical fiber probe (3) is further connected to the detector (7) through the optical fiber (5), and the detector (7) Further be connected with signal acquisition module (8), then signal acquisition module (8) is connected with A / D conversion module (9), A / D conversion module (9) is connected with further single-chip microcomputer (10), single-chip microcomputer (10) ) is co...

Embodiment 2

[0061] A device for detecting polysaccharides in fermentation broth based on Raman spectroscopy, the detection process is as follows:

[0062] (a) The incident light emitted by the exciting light source (6) enters the fermentation broth in the fermenter (1) through the optical fiber (4) and the optical fiber probe (3);

[0063] (b) The incident light is reflected by the mirror substrate, and the reflected light enters the detector (7) through the optical fiber probe (3) and the optical fiber (5);

[0064] (c) the detector (7) sends the detected Raman spectrum signal to the signal acquisition module (8);

[0065] (d) The signal collected by the signal acquisition module (8) is converted into a digital signal through the A / D conversion module (9);

[0066] (e) digital signal is transmitted to single-chip microcomputer (10);

[0067] (f) the single-chip microcomputer (10) carries out algorithmic processing to the digital signal, separates and calculates the spectrum of pure pol...

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Abstract

The invention provides a device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and a method and application thereof, and belongs to the technical field of fermentation liquor detection. The device for detecting the polysaccharide in the fermentation liquor based on the Raman spectrum comprises a fermentation tank, a reflector, an optical fiber probe, an optical fiber, an excitation light source, a detector, a signal acquisition module, an A / D conversion module, a single chip microcomputer and a display module, wherein the reflector is located in the fermentation tank, the excitation light source is connected with the optical fiber probe through an optical fiber, the optical fiber probe is connected with the detector through an optical fiber, the detector is further connected with the signal acquisition module, the signal acquisition module is connected with the A / D conversion module, the A / D conversion module is connected with the single-chip microcomputer, and the single-chip microcomputer is connected with the display module. Compared with the traditional polysaccharide detection method, the device provided by the invention does not need purification and separation, simplifies the detection steps, saves the detection time, reduces the polysaccharide detection cost, and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of fermentation liquid detection, and in particular relates to a device for detecting polysaccharides in fermentation liquid based on Raman spectroscopy, a method and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0004] There are a lot of polysaccharides in various ...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/65G01N21/01G01N2021/655G01N2021/0112
Inventor 史建国李秋顺杨俊慧马耀宏蔡雷杨艳刘庆艾孟庆军王丙莲
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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