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Bacillus subtilis subsp. Deserticola strain and application thereof

A technology of Bacillus subtilis and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of poor growth, death of the whole plant, economic loss, etc., to achieve large-scale production and popularization and application, short cultivation time, easy access to effects

Pending Publication Date: 2021-05-04
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fusarium oxysporum (Fusarium oxysporum) Fusarium oxysporum ) due to damage to the root system, the tip of the leaf is dry in light cases, the leaves are yellow-green, the growth is poor, and the whole plant dies in severe cases, causing serious economic losses

Method used

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  • Bacillus subtilis subsp. Deserticola strain and application thereof
  • Bacillus subtilis subsp. Deserticola strain and application thereof
  • Bacillus subtilis subsp. Deserticola strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Bacillus subtilis subsp. desert ( Bacillus subtilis subsp. inaquosorum ) Acquisition, identification and preservation of CPBA-W01.

[0045] (1) Bacillus subtilis desert subspecies ( Bacillus subtilis subsp. inaquosorum ) Acquisition and identification of CPBA-W01.

[0046] Bacillus subtilis desert subspecies ( Bacillus subtilis subsp. inaquosorum ), which was isolated from the coral-like mycorrhiza of Panzhihua cycads. The samples were collected from the Cycad Nature Reserve in Panzhihua City, Sichuan Province, and stored at 4°C for later use.

[0047] Isolation of endophytic bacteria from coral mycorrhizal mycorrhizae of Cycas cycads by conventional tissue method: after washing the soil on the surface of coral mycorrhizae with distilled water, the coral root tissue was cut into 0.5 cm×0.5 cm tissue pieces, and sterilized with 75% alcohol for 1 min , and then sterilized with 0.1% mercury liter solution for 5 min, and finally rinsed with sterile water for 3 ...

Embodiment 2

[0056] Bacillus subtilis subsp. desert ( Bacillus subtilis subsp. inaquosorum ) Screening of CPBA-W01 strain.

[0057] (1) Screening of strains.

[0058] Preliminary screening of antagonistic bacteria was carried out by cross method. Concrete operation is as follows: with the root rot fungus of Molan ( Fusarium oxysporum ) as an indicator bacterium, inoculate a 6 mm agar block of pathogenic bacteria in the center of the improved PDA medium plate, and then inoculate a single colony of bacteria obtained by isolation and culture at an equidistant distance of 2.5 cm from the pathogenic bacteria, inoculate 4 strains per plate, and set 5 replicates After culturing at a constant temperature of 28°C for 3-5 days, observe the size of the inhibition zone and select the strain with the best antagonistic effect.

[0059] (2) Observation of results.

[0060] The isolated strains were screened by the criss-cross method, and the results showed that there were 3 strains with antagon...

Embodiment 3

[0062] Bacillus subtilis subsp. desert ( Bacillus subtilis subsp. inaquosorum ) Determination of the biocontrol effect of CPBA-W01 strain.

[0063] (1) Determination of biocontrol effect.

[0064] Perseverance culture method: with Molan root rot ( Fusarium oxysporum ) as the indicator bacteria, first use a 6 mm puncher to punch out the activated agar block of pathogenic bacteria, put it on the plate, and then inoculate the antagonistic bacteria by spot connection method, the distance between the two bacteria is 2.5 cm, the control group is only inoculated with the agar block of pathogenic bacteria, set 3 Repeatedly, cultured in a 28°C incubator for 3-5 days, observed the growth of pathogenic bacteria, measured the inhibition zone, calculated the inhibition rate, and finally screened out the antagonistic bacteria with the best antagonistic effect. The formula for calculating the bacteriostatic rate: bacteriostatic rate (%) = (control pathogen colony diameter - treatment p...

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Abstract

The invention discloses a bacillus subtilis subsp. Deserticola strain and application thereof. The bacillus subtilis subsp. Deserticola strain is classified and named as Bacillus subtilis subsp. inaquorum, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 13th, 2020, the preservation number is 19347, and the bacillus subtilis subsp. Deserticola strain belongs to the bacteria field, phylum firmicutes, bacillus classes, bacillus families, bacillus families and bacillus sp. The application is the application of the bacillus subtilis subsp. Deserticola strain in biological prevention and control of cymbidium sinense root rot.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation and cultivation, and in particular relates to the application of a Bacillus subtilis subsp. Background technique [0002] Cymbidium( Cymbidium sinense ) is a ground plant of the genus Orchid, mainly distributed in China, India, Myanmar, Vietnam, Thailand, Japan Ryukyu Islands and other places. Molan root rot is one of the main diseases in artificial cultivation and industrial production. The disease is common and is an important disease on orchids, affecting the growth and viewing of orchids. The disease mainly affects fleshy roots. In the early stage of the disease, light brown, water-soaked lesions appeared on the roots, and after expansion, they turned brown and rotted. The roots rotted section by section until the whole root rotted, and then spread to other roots. Fusarium oxysporum (Fusarium oxysporum) Fusarium oxysporum ) due to damage to the root system, the tip of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P3/00C12R1/125
CPCC12N1/02A01N63/22
Inventor 伍建榕陈健鑫马焕成魏玉倩张东华王芳周嫒婷马翔尼玛此姆
Owner SOUTHWEST FORESTRY UNIVERSITY
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