A hairy root culture bioreactor
A bioreactor and hairy root technology, applied in the field of hairy root culture bioreactors, can solve the problems of enlarging hairy roots, accelerated aging and death of hairy roots, etc.
Active Publication Date: 2022-06-17
FOSHAN UNIVERSITY
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Problems solved by technology
[0003] At present, the cultivation of hairy roots will use the planar design of the training net in the atomization bioreactor. Due to the uneven spraying of the sprayer itself, it is easy to gather water droplets, which will increase the phenomenon that the hairy roots gather into agglomerates, resulting in internal hairy roots. Senescence accelerates or even dies, eventually affecting the cultivation of hairy roots
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[0030] The concept, specific structure and technical effects of the present invention will be clearly and completely described below with reference to the embodiments and accompanying drawings, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative efforts are all within the scope of The scope of protection of the present invention. In addition, all the coupling / connection relationships mentioned in the text do not mean that the components are directly connected, but refer to a better coupling structure by adding or reducing coupling accessories according to specific implementation conditions. Various technical features in the present invention can be combined interactively on the premise o...
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The invention discloses a hairy root cultivation bioreactor, which comprises a box body, an air intake hole, a first exhaust hole, a loading object and a spraying mechanism arranged in the box body, and a plurality of mesh holes are arranged on the object loading object. The loaded object is arranged on a curved surface, and the vertical section of the loaded object is tapered. The present invention places the plants on the object-carrying object through the mesh, and the vertical section of the object-carrying object is conical, so there is a large enough growth distance between adjacent hairy roots, which reduces the situation of hairy roots agglomerating, and sprays The water droplets formed by the mechanism for a period of time can flow down with the surface of the loading object, reducing the situation that the nutrient solution soaks the hairy roots for a long time, and contributes to the normal growth and development of the hairy roots.
Description
technical field [0001] The present invention relates to a bioreactor, in particular to a hairy root culture bioreactor. Background technique [0002] Hairy root refers to a pathological phenomenon caused by the infection of the whole plant or an organ, tissue, single cell, or even protoplast by Agrobacterium rhizogenes, which is obtained by inserting the T-DNA of the plasmid in the cell into the nuclear genome of the host cell. The phenotype, mainly on or near the infection site, can produce a large number of by-products-hairy roots, which can exhibit the ability to synthesize secondary metabolites under in vitro culture conditions. [0003] At present, the culture of hairy roots will use an atomized bioreactor. The culture net adopts a flat design. Due to the uneven spraying of the sprayer itself, it is easy to aggregate into water droplets, which increases the phenomenon of hairy roots agglomerating into lumps, resulting in internal hairy roots. Accelerated senescence and...
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IPC IPC(8): A01G31/06A01G31/02
CPCA01G31/06A01G31/02Y02P60/21
Inventor 黄丽萍郭洁茹刘力宁矫金航李帅华喻敏
Owner FOSHAN UNIVERSITY