Ischemic cerebral apoplexy screening kit and preparation method thereof

Abstract

The invention belongs to the technical field of kit preparation, and discloses an ischemic cerebral apoplexy screening kit and a preparation method. The ischemic cerebral apoplexy screening kit comprises a kit shell, a PCR assembly and a product specification, wherein the kit shell comprises an upper shell and a lower shell; and the PCR assembly comprises a PCR reaction assembly, a PCR product purification assembly and a PCR sequencing assembly. According to the invention, a method for performing PCR amplification, purification and sequencing is established, an ischemic cerebral apoplexy marker can be accurately obtained, and the PCR detection scheme is simpler and more convenient; and the method can be achieved only through blood treatment, kit reaction and post-reaction sequencing, and is more convenient than an existing laboratory PCR amplification method program. The kit is reasonable in design, the ischemic cerebral apoplexy risk can be predicted, ischemic cerebral apoplexy screening is achieved, and the screening cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of kit preparation, and in particular relates to an ischemic stroke screening kit and a preparation method. Background technique [0002] At present: According to the data released by the World Health Organization (WHO), stroke is one of the major diseases that threaten human health in the world. It is currently the primary disability and the third leading cause of death after cancer and cardiovascular disease. Epidemiology shows that the incidence of stroke is younger and increasing year by year. Stroke is mainly divided into ischemic stroke and hemorrhagic stroke according to different causes, of which ischemic stroke accounts for 87%. Studies have revealed that the main pathological mechanisms affecting the occurrence and development of stroke include energy metabolism disorders, excessive depolarization of neurons, excitotoxic injury, ion homeostasis imbalance, inflammation and immune disorders, apoptos...

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Problems solved by technology

However, the current screening method for ischemic stroke is complicated, and there is no kit that can realize ischemic stroke screening

[0003] Through the above analysis, the existing problems and defects of the existing technology are: the current screening method for ischemic stroke is complicated to operate, and there is no kit that can realize the screening of ischemic stroke

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