Method for verifying effect of SAG in preventing environmental anomaly related bone dysplasia

Abstract

The invention provides a method for verifying the effect of SAG in preventing environmental anomaly related bone dysplasia. The method comprises the following steps of changing the illumination condition in the environment, and inducing the establishment of a bone dysplasia model of an environmental rhythm disorder mouse; analyzing the bone mass and bone morphology of mandible and femur tissues of the rhythm disorder mouse, the multiplication capacity and collagen secretion capacity of corresponding cartilage cells and Hedgehog pathway related gene expression; and finally analyzing the treatment effect of the mandible and femur tissues of the mouse subjected to SAG treatment. According to the method for verifying the effect of the SAG in preventing the environmental anomaly related bone dysplasia provided by the invention, the influence of an illumination factor and time difference in the natural environment on bone growth and development can be simulated by constructing the illumination factor induced mouse bone dysplasia model, and by comparing the development conditions of the mandible and femur tissues before and after injection of the SAG, the effect of the SAG in preventing mandibular deficiency, short and deformed femur and the like can be effectively verified.

Description

technical field [0001] The invention relates to the field of medical experiments, in particular to a method for verifying that SAG prevents bone dysplasia related to environmental abnormalities. Background technique [0002] Cranio-maxillofacial dysplasia is a disease of abnormal growth and development of the cranium and jaw. The incidence rate in my country is as high as 29.3%. and severe cardiovascular and cerebrovascular diseases [Nakamura, T., et al., Noonan syndrome is associated with enhanced ERK activity, the repression of which can prevent craniofacial malformations. Proc NatlAcad Sci U S A, 2009.106(36): p.15436-41.]. At present, the main treatment method for craniofacial deformity is orthodontic correction. A conventional complete correction cycle takes 3-4 years. Deformity, the treatment of single correction method is limited, and it is easy to relapse. Therefore, exploring the pathogenesis of craniofacial deformities and realizing the early prevention of craniof...

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to seek a new method to prevent the occurrence and development of craniofacial deformities, prevent bone developmental deformities, and reduce treatment costs and risks for individuals with irregular work and rest. Corticosteroid-induced abnormal cerebellar development in mouse neonates suggests that SAG has the clinical potential to prevent bone dysplasia, but there is currently a lack of scientific methods to verify the effect of SAG in preventing environmental abnormality-related bone dysplasia

Images