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Medicine transplantation system and method for nervous tissue

A nerve tissue and medicine technology, applied in the field of nerve tissue medicine transplantation system, can solve the problems of lack of systematic nerve tissue medicine transplantation method, difficulty in obtaining and maintaining nerve tissue, limitation of neural stem cell sources, etc. Proliferating and implementable effects

Pending Publication Date: 2021-05-14
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The position of adult neural stem cells is relatively deep, it is difficult to obtain materials, and it is not easy to obtain neural stem cells, and obtaining neural stem cells from living tissues is dangerous, and the source of neural stem cells for transplantation is limited
[0006] (2) It is difficult to obtain a tissue that maintains the layer structure and function reflecting the nervous tissue of a human body in the prior art, and it is difficult to routineize it as a treatment, and there is a lack of a systematic medical transplantation method for nervous tissue

Method used

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  • Medicine transplantation system and method for nervous tissue
  • Medicine transplantation system and method for nervous tissue
  • Medicine transplantation system and method for nervous tissue

Examples

Experimental program
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Effect test

Embodiment 1

[0083] The medical transplantation method for nerve tissue provided by the embodiment of the present invention is as follows: figure 1 As shown, as a preferred embodiment, such as image 3 As shown, the mononuclear cells to be cultured are prepared into a cell suspension with DMEM culture medium containing 15-20% fetal bovine serum through the cell proliferation culture module provided in the embodiment of the present invention, and then placed in an incubator for mononuclear cell suspension. Proliferation and culture of cells, including:

[0084] S201, prepare the mononuclear cells to be cultured into a cell suspension with DMEM culture medium containing 15-20% fetal bovine serum through the cell proliferation culture module, and inoculate them into culture flasks;

[0085] S202, place the culture flask in 5% CO 2 Cultivate in an incubator, and digest with 0.25% trypsin when the adherent cells cover the bottom of the bottle;

[0086] S203, after trypsinization for 20-30 mi...

Embodiment 2

[0088] The medical transplantation method for nerve tissue provided by the embodiment of the present invention is as follows: figure 1 As shown, as a preferred embodiment, such as Figure 4As shown, in the nerve tissue screening and purification module provided in the embodiment of the present invention, the proliferated and cultured tissue cells are prepared into a cell suspension with DMEM / F12 serum-free culture medium, and then placed in an incubator to continue culturing to obtain nerve tissue-oriented stem cells, including:

[0089] S301, after the cells in the culture flask are terminated by 15-20% fetal bovine serum DMEM culture solution, the tissue cells after proliferation and culture are treated with 2% B27, 1% N 2 , 20ng / ml bFGF, 20ng / ml EGF DMEM / F12 serum-free medium to make cell suspension;

[0090] S302, placed in 5% CO 2 Cultivate in the incubator until cell spheres appear, take a larger cell sphere, and use the DMEM / F12 serum-free medium to make a cell suspe...

Embodiment 3

[0093] The medical transplantation method for nerve tissue provided by the embodiment of the present invention is as follows: figure 1 As shown, as a preferred embodiment, such as Figure 5 As shown, the engineered neural tissue construction module provided by the embodiment of the present invention utilizes the construction program to control the construction equipment and utilizes the neural tissue-directed stem cell suspension and collagen / Matrigel scaffold material to construct engineered neural tissue, including:

[0094] S401, mix the collagen and 10×DMEM evenly, adjust the pH value to 7.2-7.4 with NaOH, add Matrigel, mix evenly again, and obtain the collagen / Matrigel scaffold material;

[0095] S402, mixing the prepared neural tissue-directed stem cell suspension with the collagen / Matrigel scaffold material to obtain a cell-scaffold material composite;

[0096] S403, adding the obtained cell-scaffold complex to the culture plate, placed in 5% CO 2 , Cultivate in an in...

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Abstract

The invention belongs to the technical field of biological tissue engineering, and discloses a medicine transplantation system and method for nervous tissue. The medicine transplantation system for the nervous tissue comprises a cell proliferation culture module, a nervous tissue screening and purifying module, a nervous tissue culture module, a central control module, an engineered nervous tissue construction module, a nervous tissue storage module, a medicine transplantation module, a detection and evaluation module, a data storage module and an update display module. According to the medicine transplantation system for the nervous tissue, the collagen and the Matrigel matrigel serve as a scaffold of the tissue-engineered nervous tissue, and compared with an existing scaffold material for the nervous tissue, the scaffold material can effectively promote survival of cells in the material. The medicine transplantation system is simple to operate and high in practicability, can better maintain the survival and proliferation of the directional stem cells of the nervous tissue and promote the differentiation of the neural stem cells, and has important significance in promoting the development of the tissue-engineered nervous tissue.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering, and in particular relates to a medical transplantation system and method for nerve tissue. Background technique [0002] Currently, in the nervous tissue of an organism, one or more types of nervous system cells form a layer structure. Retinal tissue, which is one of nerve tissues, is mainly composed of five types of nerve cells, namely visual cells, bipolar cells, horizontal cells, amacrine cells, and ganglion cells, as well as glial cells, forming a three-dimensional layer structure. As a treatment for neurological diseases such as retinal degenerative diseases, it has been suggested that transplantation therapy using nerve tissue is effective. [0003] The study of cell transplantation has brought broad prospects for the treatment of neurological diseases such as brain and spinal cord trauma, cerebral ischemia, Parkinson's disease and other degenerative diseases, as well...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/50A61L27/54C12N5/0786C12N5/0797
CPCA61L27/3834A61L27/3878A61L27/3895A61L27/24A61L27/50A61L27/54C12N5/0645C12N5/0623C12N2509/00A61L2430/32A61L2300/414A61L2430/40
Inventor 李褒曼夏茂盛龚文亮陈斌杰纪明张曼曼陈蓓娜张殿君李欣宇
Owner 中国医科大学
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