Micro-drop digital polymerase chain reaction chip

Abstract

The invention discloses a microdroplet type digital polymerase chain reaction chip. The chip comprises a microdroplet structure, a microdroplet collection structure and a flow channel structure; the microdroplet structure forms a sample cavity and an oil storage cavity which are spaced, the microdroplet collection structure comprises a microdroplet collection tube arranged below the microdroplet structure, a collection cavity is formed in the droplet collection pipe, the flow channel structure is arranged on the droplet structure, an outlet of the flow channel structure is communicated with the collection cavity, inlets of the flow channel structure comprise a first inlet and a second inlet, the first inlet is communicated with the lower end of the oil storage cavity, and the second inlet is communicated with the lower end of the sample cavity; an inlet of the flow channel structure is respectively communicated with the lower end of the sample cavity and the lower end of the oil storage cavity, so that a sample and an oil product can enter the flow channel structure under the action of gravity, micro-droplets wrapped with the sample are formed under the action of the flow channel structure, and an outlet of the flow channel structure is communicated with a collecting cavity, so that the micro-droplets flow into the collecting cavity through the flow channel structure; and pipetting is not needed in the whole process.

Description

technical field [0001] The invention relates to the technical field of polymerase chain reaction, in particular to a droplet type digital polymerase chain reaction chip. Background technique [0002] Polymerase Chain Reaction (PCR) is a molecular biology technique that can amplify specific DNA fragments in vitro, and is widely used in basic biological research, clinical diagnosis, genomics, microbial detection, forensic science, etc. field. Digital PCR is a third-generation PCR technology developed based on traditional PCR technology. The basic principle is to distribute a standard PCR reaction into a large number of tiny reaction units, and each reaction unit contains or does not contain one or more copies of the target Molecule (DNA template), perform PCR amplification on the target molecule, perform statistical analysis on the fluorescent signal of the reaction unit after PCR amplification, and calculate the template copy number in the original sample. Digital PCR techn...

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Problems solved by technology

[0004] The main purpose of the present invention is to propose a micro-droplet digital polymerase chain reaction chip, aiming to solve the problem that the existing micro-droplet digital polymerase chain reaction system is not highly integrated

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