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Production method of fructus cannabis meal protein polypeptide liquid, polypeptide liquid with uric acid reducing effect and application of polypeptide liquid

A technology of hemp seed meal and production method, which is applied in the field of polypeptide preparation with uric acid-lowering function, and can solve the problems of waste of resources and insufficient deep processing, etc.

Active Publication Date: 2021-05-18
天津北洋百川生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, hemp seed meal is mostly used as feed, and the deep processing is insufficient, resulting in a great waste of resources

Method used

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  • Production method of fructus cannabis meal protein polypeptide liquid, polypeptide liquid with uric acid reducing effect and application of polypeptide liquid
  • Production method of fructus cannabis meal protein polypeptide liquid, polypeptide liquid with uric acid reducing effect and application of polypeptide liquid
  • Production method of fructus cannabis meal protein polypeptide liquid, polypeptide liquid with uric acid reducing effect and application of polypeptide liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Mix the hemp seed meal and water according to the material-to-liquid ratio of 1:10, pass through a colloid mill to crush and mix evenly, and obtain the hemp seed meal stock solution; prepare the above stock solution to a suitable pH value of 7.5, put it into an ultrasonic cleaning machine, Power 400w, treat for 20 minutes; after inactivating the enzyme, add 12000U / g ([E] / [S]) alkaline protease to the protein stock solution, mix well, enzymolyze at 50°C for 5 hours, inactivate the enzyme, and obtain a one-step enzyme Polypeptide solution; 4000r / min centrifugal 10min can get HSPS1 (referring to the Hemp Seed Polypeptide Solution processed in Example 1).

[0074] The peak shape and peak area of ​​HSPS1 uric acid determined by RP-HPLC are as follows figure 1 .

Embodiment 2

[0076] Mix the hemp seed meal and water according to the material-to-liquid ratio of 1:10, pass through a colloid mill to crush and mix evenly, and obtain the hemp seed meal stock solution; prepare the above stock solution to a suitable pH value of 7.5, put it into an ultrasonic cleaning machine, Power 400w, treat for 20 minutes; after inactivating the enzyme, add 12000U / g ([E] / [S]) alkaline protease to the protein stock solution, mix well, enzymolyze at 50°C for 5 hours, inactivate the enzyme, and obtain a one-step enzyme Polypeptide hydrolysis liquid; pasteurize the one-step enzymatic hydrolysis polypeptide liquid, insert 2% Lactobacillus bulgaricus, anaerobically ferment at 43°C for 8h, and ferment at 4°C for 24h; sterilize, centrifuge at 4000r / min for 10min to obtain HSPS2 (refer to What is treated in embodiment 2 is the hemp seed polypeptide liquid that obtains).

[0077] The peak shape and peak area of ​​HSPS2 uric acid determined by RP-HPLC are as follows figure 1 .

Embodiment 3

[0079] Mix the hemp seed meal and water according to the material-to-liquid ratio of 1:10, pass through a colloid mill to crush and mix evenly, and obtain the hemp seed meal stock solution; prepare the above stock solution to a suitable pH value of 7.5, put it into an ultrasonic cleaning machine, Power 400w, treat for 20 minutes; after inactivating the enzyme, add 12000U / g ([E] / [S]) alkaline protease to the protein stock solution, mix well, enzymolyze at 50°C for 5 hours, inactivate the enzyme, and obtain a one-step enzyme Polypeptide hydrolysis solution; pasteurize the one-step enzymatic hydrolysis polypeptide solution and insert 2% Lactobacillus delbrueckii, anaerobically ferment at 43°C for 8h, and ferment at 4°C for 24h; sterilize, centrifuge at 4000r / min for 10min to obtain HSPS3( Refer to the hemp seed polypeptide liquid obtained by processing in embodiment 3).

[0080] The peak shape and peak area of ​​HSPS3 uric acid determined by RP-HPLC are as follows figure 1 .

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Abstract

The invention discloses a production method of a fructus cannabis meal protein polypeptide liquid and the polypeptide liquid with a uric acid reducing effect. The production method of the fructus cannabis meal protein polypeptide liquid comprises the following steps: adding water into fructus cannabis meal, carrying out pulping, performing ultrasonic treatment, adjusting the pH value, then adding alkaline protease, performing hydrolysis, then performing enzyme deactivation to obtain a fructus cannabis one-step enzymatic hydrolysate, and implementing an enzymolysis combined fermentation method or a two-step enzymolysis method to obtain a group of bioactive polypeptide liquid with the uric acid reducing function. The in-vitro xanthine oxidase inhibitory activity of polypeptide liquid is measured through reverse high performance liquid chromatography, commonly used allopurinol is used as positive control and compared with the fructus cannabis meal polypeptide liquid, and the result shows that the bioactive peptide prepared from the fructus cannabis meal by combining enzymolysis and biological fermentation technologies is remarkable in uric acid reducing effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing a polypeptide with a function of reducing uric acid and its application. Background technique [0002] Gout, also known as hyperuricemia, is a disease caused by the disorder of purine metabolism in the body and the increase of uric acid. In recent years, the proportion of people eating high-energy and high-purine foods has increased significantly; and as the pace of life continues to accelerate, the human body is often in a state of stress, and the internal environment is disordered, and a large amount of purine will be produced in the body; coupled with irregular daily life , lack of physical exercise, affecting the excretion of uric acid. So that hyperuricemia has become a common and frequently-occurring disease. [0003] The current treatment for hyperuricemia is mainly to use colchicine or non-steroidal anti-inflammatory drugs to reduce inflammation durin...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L33/18A61K38/01A61P19/06C12R1/25C12R1/225
CPCC12P21/06A23L33/18A61K38/011A61P19/06A23V2002/00A23V2250/55A23V2200/30
Inventor 乔长晟张泽宇牛思思钟海蛟于世云孙银华
Owner 天津北洋百川生物技术有限公司
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