Dual-wavelength dual-scale nano-drug living body imaging system and time sequence control method
A nano-drug, in vivo imaging technology, applied in the field of nano-drug fluorescence microscopy imaging, can solve the problems of inability to evaluate metabolic organ damage, inability to meet cell imaging, inability to achieve nano-drug transport characteristics, etc.
Active Publication Date: 2021-05-18
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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Problems solved by technology
Although various in vivo imaging systems (CT / SPECT / US / MRI / PET, etc.) can show that nanomedicines are more concentrated in diseased tissues and organs, their spatial resolution is often at the millimeter level, which is far from meeting the needs of cells in vivo. The need for imaging and the inability to evaluate the damage of major metabolic organs
Although various optical microscopy techniques (laser confocal microscopy, two-photon microscopy, etc.) have very good resolution and can microscopically monitor the characteristics of cellular structures and even subcellular structures, they are usually only used for isolated cells. Or tissue observation, can not realize the study of the transport properties of nano-drugs in the target cells of living animals
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[0033] Such as figure 1 As shown, a dual-wavelength dual-scale nanomedicine in vivo imaging system in this embodiment includes an illumination unit, a microscopic imaging unit and a PC host computer, and the microscopic imaging unit includes a tissue imaging submodule and a target cell imaging submodule , the lighting unit is used to emit the first excitation light and / or the second excitation light, the tissue imaging submodule obtains the first excitation light or the second excitation light to obtain the mesoscopic distribution image of the nanomedicine, and the target cell imaging submodule obtains the first excitation light light and the second excitation light to obtain the microscopic distribution image of the nanomedicine at the cell level, and the host computer controls the lighting unit to periodically emit the first excitation light and / or the second excitation light and control the images of the tissue imaging submodule and the target cell imaging submodule collect...
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Abstract
The invention discloses a dual-wavelength dual-scale nano-drug living body imaging system which comprises a lighting unit, a microscopic imaging unit and a PC upper computer. The microscopic imaging unit comprises a tissue imaging submodule and a target cell imaging submodule, the lighting unit is used for emitting first exciting light and / or second exciting light, the tissue imaging submodule obtains the first exciting light or the second exciting light to obtain a mesoscopic distribution image of a nano-drug, and the target cell imaging submodule obtains the first exciting light and the second exciting light to obtain a microscopic distribution image of the nano-drug on a cellular level. Synchronous information of distribution and damage of main metabolic organs of mesoscopic-level nano-drug and distribution and transport characteristics of microcosmic-level monitoring nano-drug in target cells can be obtained in the same living small animal body; and the upper computer uses time sequence accurate control, reduces random errors of measurement of different animals at different time, and clarifies the transfer mechanism of the nano-drug in the small animal body. The invention further discloses a time sequence control method of the imaging system.
Description
technical field [0001] The invention relates to fluorescence microscopic imaging of nano-medicine, in particular to a dual-wavelength and dual-scale nano-medicine live imaging system and a timing control method thereof. Background technique [0002] Compared with traditional drugs, nano-drugs have the advantages of long plasma half-life, low clearance rate, and targeted drug delivery to diseased tissues, which can effectively improve the therapeutic effect of drugs and reduce side effects. At the same time, the change of the transmembrane mechanism can increase the permeability of the drug to the biomembrane, which is conducive to the tissue absorption of the drug and the exertion of the intracellular drug effect, thereby improving the curative effect of drug-resistant diseases. It has become a hot spot in current drug research. [0003] However, the druggability of nanomedicines, such as effectiveness, safety, and quality controllability, have always been the bottlenecks t...
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IPC IPC(8): G01N21/64
CPCG01N21/6458
Inventor 陶玲王飞龙李韪韬钱志余童云坤周慧晶李怡燃姚柳叶
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS