A dual-wavelength dual-scale nano-drug in vivo imaging system and timing control method

Abstract

The invention discloses a dual-wavelength dual-scale nano-drug in vivo imaging system, comprising an illumination unit, a microscopic imaging unit and a PC upper computer. The microscopic imaging unit includes a tissue imaging sub-module and a target cell imaging sub-module, and the illumination unit is used for emitting The first excitation light and / or the second excitation light, the tissue imaging sub-module obtains the first excitation light or the second excitation light to obtain the mesoscopic distribution image of the nanomedicine, and the target cell imaging sub-module obtains the first excitation light and the second excitation light Obtain the microscopic distribution image of nanomedicine at the cellular level. The distribution and damage of the main metabolizing organs of nano-drugs at the mesoscopic level and the synchronization information of monitoring the distribution and transport characteristics of nano-drugs in target cells at the microscopic level can be obtained in the same living small animal; Random errors of measurements in different animals, elucidating the transport mechanism of nanomedicines in small animals. The invention also discloses a timing control method of the above imaging system.

Description

technical field [0001] The invention relates to nanometer drug fluorescence microscopic imaging, in particular to a dual-wavelength dual-scale nanometer drug in vivo imaging system and a time sequence control method thereof. Background technique [0002] Compared with traditional drugs, nano-drugs have the advantages of long plasma half-life, low clearance rate, targeted drug delivery to diseased tissues, etc., which can effectively improve the therapeutic effect of drugs and reduce toxic and side effects. At the same time, the change of the transmembrane mechanism can increase the permeability of the drug to the biomembrane, which is beneficial to the tissue absorption of the drug and the exertion of the intracellular drug effect, thereby improving the curative effect of drug-resistant diseases. Nano-drugs have their unique advantages. become the focus of current drug research. [0003] However, the druggability of nano-drugs, such as efficacy, safety, and quality control,...

AI Technical Summary

Problems solved by technology

Although various in vivo imaging systems (CT / SPECT / US / MRI / PET, etc.) can show that nanomedicines are more concentrated in diseased tissues and organs, their spatial resolution is often at the millimeter level, which is far from meeting the needs of cells in vivo. The need for imaging and the inability to evaluate the damage of major metabolic organs

Although various optical microscopy techniques (laser confocal microscopy, two-photon microscopy, etc.) have very good resolution and can microscopically monitor the characteristics of cellular structures and even subcellular structures, they are usually only used for isolated cells. Or tissue observation, can not realize the study of the transport properties of nano-drugs in the target cells of living animals

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