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Alkali-resistant SNP (Single Nucleotide Polymorphism) marker for momulberry bike

A tilapia, alkali-resistant technology, applied in climate change adaptation, recombinant DNA technology, DNA/RNA fragments, etc., to achieve the effect of great scientific value and commercial value

Active Publication Date: 2021-05-25
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the detection or screening of Mozambique tilapia using SNP marker technology

Method used

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  • Alkali-resistant SNP (Single Nucleotide Polymorphism) marker for momulberry bike
  • Alkali-resistant SNP (Single Nucleotide Polymorphism) marker for momulberry bike
  • Alkali-resistant SNP (Single Nucleotide Polymorphism) marker for momulberry bike

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Study on the action of SNP tag of this example:

[0040]The Mozambian Mossambicus (500 tails) used in this example comes from the high demanded aquatic breeding base of the Zhujiang Aquatic Research Institute, China Aquatic Science Research Institute. The alkali stress experiment is carried out in a fish pool of 320 cm × 200 cm × 150 cm. Relaying 7 days before the experiment, dissolved oxygen during 8.7 ± 0.6mg / L, water temperature is 28.5 ± 1 ℃, pH value 8.4 ± 0.5. Choose a healthy and hurtful, lively tilapia, after the experiment, add pre-pre-pre-pre-pre-pre-pre-predicted sodium bicarbonate every 20 minutes (Nahco3The solution increases the carbonate alkali of the pool water, and the alkalinity ratio of 1 g / L is increased each time, the actual carbonate degree of the water is measured with acid-base titration, titration with 1 mol / L hydrochloric acid solution (HCl), and methyl orange The actual carbonate level of the pool water is detected in the indicator. During the per...

Embodiment 2

[0046]This embodiment of the SNP markers Mozambique tilapia screening / detection:

[0047]Extraction (a) sample DNA

[0048](1) Retrieving the test sample Mozambique non-fish fin tissue 3 mg, after cutting, 0.5 ml of lysate (10 mmol / L Tris-HCl; 0.1 mol / L EDTA; 0.5% SDS; 30 mg / L RNase; 100 mg / L protease K, pH 8.0), digested at 55 ° C for 1 hour;

[0049](2) Add equal volume of phenol / chloroform (volume ratio 25: 24: 1) mixture, mix, after standing for 5 minutes, 12000 rpm, centrifuge for 10 minutes, take the supernatant, and then Take it once with chloroform, after standing for 5 minutes at room temperature, centrifugation 12000 rpm for 10 minutes, take the supernatant;

[0050](3) 2x volume of anhydrous ethanol was added, and it was allowed to stand for 10 minutes to precipitate DNA, and 12000 rpm was centrifuged for 10 minutes to obtain a precipitate;

[0051](4) The precipitate was washed once with 70% ethanol, 12000 rpm for 2 minutes, suction of the supernatant, and allowed to stand ...

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PUM

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Abstract

The invention belongs to the technical field of aquatic organisms, and discloses an alkali-resistant SNP (Single Nucleotide Polymorphism) marker of momulberry bike tilapia and application of the alkali-resistant SNP marker. The sequence of the SNP marker is shown as SEQ ID NO: 1 or SEQ ID NO: 4, and the base of an SNP site at the 1227bp position of the SEQ ID NO: 1 sequence or the 120bp position of the SEQ ID NO: 4 sequence is G or A. The invention creatively provides the SNP marker related to the alkali-resistant character of the momulberry bike tilapia, and develops the corresponding primer pair, the method and the kit on the basis of the SNP marker, so that the alkali-resistant individual of the momulberry bike tilapia can be screened or detected without being limited by the growth stage; the method can greatly improve the breeding process of tilapia mossambica and quickly select tilapia mossambica germplasm with excellent alkali-resistant character, and has great scientific value and commercial value.

Description

Technical field[0001]The present invention belongs to the field of aquatic biological technology, and specific involves a Mozambian Croo non-cosmetic SNP marker and its application thereof.Background technique[0002]Tilapia, originally produced in Africa, commonly known as African squid, a spine tropical fish in a whitelidae (Cichlidae). As an important economic fish, tilapia has the characteristics of fresh meat, high nutritional value, fast growing, and is favored by consumers and farmers, has been listed as six major foods by the United Nations FAO (FAO). First, farming has been carried out in more than 100 countries and regions. At present, there are a variety of cultured varieties such as Mozambarian non-fish, Nile ruthenium, Olia Ribfish and hybrid, and different range of salt resistance.[0003]The Mozambian Mossambicus is called Vietnamese fish in the 1950s. The origin of Mozambique in Africa, from 1956, in 1957, Thailand and Vietnamese were piloted in Guangdong Province in Gua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113Y02A40/81
Inventor 朱华平马冬梅樊佳佳苏换换李娅娅
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI