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SNP marker related to salt tolerance of tilapia mossambica and application of SNP marker

A tilapia and salt-tolerant technology, which can be applied in climate change adaptation, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of low selection rate and long cycle, and achieve good specificity, great scientific value and commercial value. The effect of high value and amplification efficiency

Active Publication Date: 2021-06-22
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are problems such as low selection rate and long cycle in the selection of salt-tolerant tilapia by traditional breeding methods

Method used

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  • SNP marker related to salt tolerance of tilapia mossambica and application of SNP marker
  • SNP marker related to salt tolerance of tilapia mossambica and application of SNP marker
  • SNP marker related to salt tolerance of tilapia mossambica and application of SNP marker

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Experimental program
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Embodiment 1

[0037] Research on the role of SNP markers of the present invention:

[0038]The Oreochromis hornorum (500 tails) used in the present invention all come from the Gaoyao Aquatic Germplasm Base of the Zhujiang Fisheries Research Institute of the Chinese Academy of Fishery Sciences. The salinity stress experiment was carried out in a fish pond of 320cm×200cm×150cm. Before the experiment, they were temporarily raised for 7 days, during which the dissolved oxygen was 8.7±0.6mg / L, the water temperature was 28.5±1°C, and the pH value was 8.4±0.5. Select healthy, non-injured and lively tilapia for the experiment. After the experiment starts, add pre-prepared salt water of about 113‰ every 30 minutes to increase the salinity of the pool water, and increase the salinity by 5‰ each time. Use a hand-held salinity meter Detects the actual salinity of the pool water. During the continuous inflation, no feed was fed. After 2 hours, tilapia began to die, and the death time, water salinity,...

Embodiment 2

[0044] This embodiment screens / detects the SNP markers of tilapia:

[0045] (1) Extraction of sample DNA

[0046] (1) Take 3 mg of the fin ray tissue of the tilapia sample to be tested, cut it into pieces, and add 0.5 mL of lysate (10 mmol / L Tris-HCl; 0.1 mol / L EDTA; 0.5% SDS; 30 mg / L RNase; 100 mg / L L proteinase K, pH8.0), digested at 55°C for 1 hour;

[0047] (2) Add an equal volume of phenol / chloroform / isoamyl alcohol (volume ratio 25:24:1) mixture, mix well, let stand at room temperature for 5 minutes, centrifuge at 12000 rpm for 10 minutes, take the supernatant, and then Extract once with chloroform, let stand at room temperature for 5 minutes, centrifuge at 12,000 rpm for 10 minutes, and take the supernatant;

[0048] (3) Add 2 times the volume of absolute ethanol, let stand at room temperature for 10 minutes to precipitate DNA, and centrifuge at 12,000 rpm for 10 minutes to obtain a precipitate;

[0049] (4) The precipitate was washed once with 70% ethanol, centrifug...

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Abstract

The invention belongs to the technical field of aquatic organisms, and discloses an SNP (Single Nucleotide Polymorphism) marker related to a salt-tolerant character of tilapia and application of the SNP marker. The sequence of the SNP marker is shown as SEQ ID NO: 1 or SEQ ID NO: 4, and the basic group of an SNP site at the 2942bp position of the SEQ ID NO: 1 sequence or the 99bp position of the SEQ ID NO: 4 sequence is G or T. The invention creatively provides the SNP marker related to the salt-tolerant character of the tilapia mossambica, on the basis, the SNP marker can be used for screening or detecting salt-tolerant tilapia mossambica individuals and is not limited by the growth stage, the breeding process of the tilapia mossambica can be greatly improved, and the tilapia mossambica germplasm with the excellent salt-tolerant character can be quickly selected; a primer pair and a kit for corresponding detection are developed on the basis, the genotype of the tilapia mossambica to be detected can be quickly judged, and great scientific value and commercial value are achieved.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a SNP marker related to the salt tolerance of tilapia and an application thereof. Background technique [0002] Tilapia, native to Africa, commonly known as African crucian carp, is a euryhalal tropical fish belonging to the family Cichlidae of the order Perciformes. As an important economic fish, tilapia has the characteristics of fresh and tender meat, high nutritional value, and fast growth. It is favored by consumers and farmers and has been listed as one of the six staple foods by the Food and Agriculture Organization of the United Nations (FAO). 1. Breeding has been carried out in more than 100 countries and regions. my country is the world's largest producer and consumer of tilapia. In 2019, the output reached 1.6417 million tons, accounting for about 60% of the world's tilapia production, mainly including Mozambique tilapia, Nile tilapia, Ollie For ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02A40/81
Inventor 朱华平马冬梅樊佳佳苏换换
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI