Streptomyces secretory expression vector as well as construction method and application thereof

Abstract

The invention discloses a streptomyces secretory expression vector as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The invention provides a streptomyces secreting type expression vector. The streptomyces secreting type expression vector comprises a sequence as shown in SEQ ID NO. 1. The expression vector is specifically prepared by the following steps: fusing a strong promoter PA4 and a specific secretion signal peptide with the N end of a target gene to be expressed, and inserting into a conventional vector to construct a recombinant plasmid, so that the target gene can be expressed, and the expressed target protein can be secreted outside a cell; in the fermentation culture process of the recombinant strain, the secretory-expressed exogenous target protein can be directly collected from the fermentation liquor. The construction method of the vector is simple and rapid, and the target protein can be directly secreted outside the cell, so that the collection of the target protein is facilitated, and the cytotoxicity caused by heterologous protein accumulation is avoided.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a streptomyces secreted expression vector and its construction method and application. Background technique [0002] Protein products such as enzymes, hemoglobin, and antibodies are in great demand in daily life. The low level of protein expression from natural sources severely limits the development of protein products. With the rise of genetic engineering technology, the development of heterologous expression systems has effectively increased the production of proteins, making recombinant proteins currently used in chemical, fuel, and food industries. And other fields already have a relatively high market share, especially biomedical proteins contain great production potential and commercial value. [0003] When expressing heterologous proteins, choosing an appropriate expression system is critical. Escherichia coli expression system was first develope...

AI Technical Summary

Problems solved by technology

Commonly used E. coli and yeast expression systems often do not work well for expressing proteins from specific sources such as Streptomyces or Archaea

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