Method for purifying euglena on agar plate

Abstract

The invention provides a method for purifying euglena on an agar plate, and belongs to the technical field of euglena purification. The method comprises the following steps: culturing a to-be-purified water sample containing euglena cells under an illumination condition, collecting phototaxis enriched euglena, centrifuging, and collecting precipitate; and applying the light to the side surface of the algae liquid level of the water sample to be purified, wherein the horizontal included angle between the light of the light and the algae liquid level of the water sample to be purified is less than or equal to 60 degrees. The enriched euglena is firstly subjected to plate dripping point purification, the euglena can grow up quickly and has interspecific advantages, and after the euglena grows out, the euglena is subjected to streak purification, so that the purification efficiency of the sterile euglena can be improved.

Description

technical field [0001] The invention relates to the technical field of euglena purification, in particular to a method for purifying euglena on an agar plate. Background technique [0002] Euglena has both animal and plant characteristics. Euglena is the best experimental material for studying endosymbiosis, chloroplast development and photosynthesis. At the same time, euglena has high commercial value. Euglena is rich in nutrients: vitamins, mineral nutrients, amino acids, unsaturated fatty acids, chlorophyll, lutein, zeaxanthin and other 59 kinds of nutrients necessary for the human body every day, all of which are indispensable nutrients for maintaining health; the most important The Euglena component of Euglena is a unique component of Euglena. Euglena can inhibit the absorption of purine, and the use of natural Euglena to treat gout has also been realized. In Japan, which likes to eat seafood, it has obtained an invention patent. See Japanese Morinaga Pharmaceutical ...

AI Technical Summary

Problems solved by technology

In terms of euglena purification, for example, patent CN108277163A discloses a method for separating and purifying euglena species, and patent CN107686814A discloses a method for separating and purifying euglena species on a flat solid medium. The enrichment and purification method of euglena, however, this method can only be enriched and purified according to the acid resistance, phototropism and antibiotic resistance of euglena, and it is impossible to obtain completely sterile algae species

After enrichment of euglena by the method in the prior art, the sample still contains more bacteria. The traditional method is to purify euglena by using antibiotic plate streaking method, but this method requires higher purity of euglena , which is difficult to achieve in actual operation, often the bacteria grow out first and cover the growth of euglena, and it is difficult to obtain a single euglena algae colony

In addition, the patent CN 108277163 A discloses a method for separating and purifying euglena species. The patent discloses that the lower part of the test tube is covered with an opaque material, and the upper part is irradiated with a light intensity of 3000 lx for 30 minutes to make the euglena phototaxis toward the upper part of the test tube. collection, this method has higher requirements on the light intensity, which must be completely suitable for the growth of euglena, so that the euglena will gather in the upper part; in addition, the upward movement of euglena needs to provide its own power, which will affect the activity of euglena. The requirements are relatively high. If the euglena is not active and cannot swim upward, it is difficult to realize this scheme in actual operation.

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