Preparation method of time-resolved fluorescence immunoassay quantitative chromatography test strip for plasticizer in food

A time-resolved fluorescence and immunoquantitative technology, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the problems of unsatisfactory food supervision, low plasticizer content, limited production control, etc., to improve loading quality and high accuracy , Improve the effect of detection ability

Pending Publication Date: 2021-06-01
上海雄图生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plasticizer is a polymer material additive, the most common species is bis(2-ethylhexyl) phthalate, which is a colorless, odorless liquid, although it is expressly stipulated in food that plasticizers are not allowed It is not allowed to be added to food, and food packaging is not allowed, but due to limited production control and the influence of environmental factors, plasticizer pollution generally exists in food
The content of plasticizers in food is often very low, which brings a huge test to the detection, and only qualitative detection can not meet the food supervision. How to accurately detect the content of plasticizers in food is an urgent problem to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for making plasticizer time-resolved fluorescent immunoquantitative chromatography test strips in food includes: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively. The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:

[0034] Step 1. Draw different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions and spray them onto the microsphere areas of multiple test strips, place at 10°C, and treat with negative pressure for 15 minutes;

[0035] Step 2, absorb different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spray them on the detection line a...

Embodiment 2

[0045] The method for making time-resolved fluorescent immunoquantitative chromatography test strips for plasticizers in food comprises: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively, and The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:

[0046] Step 1. Spray different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions onto the microsphere areas of multiple test strips, place at 20°C, and treat with negative pressure for 30 minutes;

[0047] Step 2, drawing different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spraying them on the detection line a...

Embodiment 3

[0057] The method for making time-resolved fluorescent immunoquantitative chromatography test strips for plasticizers in food comprises: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively, and The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:

[0058] Step 1. Spray different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions on the microsphere areas of multiple test strips, place at 15°C, and treat with negative pressure for 20 minutes;

[0059] Step 2, absorb different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spray them on the detection line and qua...

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Abstract

The invention discloses a preparation method of a time-resolved fluorescence immunoassay quantitative chromatography test strip for a plasticizer in food. According to the method, a reaction film is fixed on a substrate, a sample absorption pad and a water absorption pad are respectively overlapped at two ends of the reaction film; a sample adding area and a microsphere area are arranged on the sample absorption pad at an interval; and a detection line and a quality control line are arranged on the reaction film. The method comprises the following steps of: step 1, preparing a time-resolved fluorescent microsphere solution of which the surface is coated with an anti-plasticizer monoclonal antibody; step 2, preparing a plasticizer-bovine serum albumin conjugate solution and a goat anti-mouse IgG secondary antibody solution; 3, spraying the time-resolved fluorescent microsphere solution to the microsphere area, and carrying out negative pressure treatment; 4, respectively spraying the anti-plasticizer monoclonal antibody solution and the goat anti-mouse IgG secondary antibody solution onto the detection line and the quality control line; 5, performing ultrasonic treatment; and 6, drying a product in a drying oven. The test strip prepared by the method can quickly and accurately detecting the content of the plasticizer.

Description

technical field [0001] The invention relates to the technical field of plasticizer detection. More specifically, the present invention relates to a preparation method of time-resolved fluorescent immunoquantitative chromatography test strips for plasticizers in food. Background technique [0002] Plasticizer is a polymer material additive, the most common species is bis(2-ethylhexyl) phthalate, which is a colorless, odorless liquid, although it is expressly stipulated in food that plasticizers are not allowed It is not allowed to be added to food, and food packaging is not allowed, but due to limited production control and the influence of environmental factors, plasticizer pollution generally exists in food. The content of plasticizers in food is often very low, which brings a huge test to the detection, and only qualitative detection can not meet the food supervision. How to accurately detect the content of plasticizers in food is an urgent problem to be solved. Content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/577
CPCG01N33/533G01N33/577
Inventor 汪劲能
Owner 上海雄图生物科技有限公司
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