Preparation method of time-resolved fluorescence immunoassay quantitative chromatography test strip for plasticizer in food
A time-resolved fluorescence and immunoquantitative technology, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the problems of unsatisfactory food supervision, low plasticizer content, limited production control, etc., to improve loading quality and high accuracy , Improve the effect of detection ability
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Embodiment 1
[0033] The method for making plasticizer time-resolved fluorescent immunoquantitative chromatography test strips in food includes: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively. The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:
[0034] Step 1. Draw different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions and spray them onto the microsphere areas of multiple test strips, place at 10°C, and treat with negative pressure for 15 minutes;
[0035] Step 2, absorb different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spray them on the detection line a...
Embodiment 2
[0045] The method for making time-resolved fluorescent immunoquantitative chromatography test strips for plasticizers in food comprises: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively, and The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:
[0046] Step 1. Spray different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions onto the microsphere areas of multiple test strips, place at 20°C, and treat with negative pressure for 30 minutes;
[0047] Step 2, drawing different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spraying them on the detection line a...
Embodiment 3
[0057] The method for making time-resolved fluorescent immunoquantitative chromatography test strips for plasticizers in food comprises: fixing a reaction film on a substrate, and then lapping a sample absorption pad and a water absorption pad on both ends of the reaction film respectively, and The sample absorption pad is provided with a sample loading area and a microsphere area at intervals, and the reaction film is provided with a detection line and a quality control line, which is characterized in that it includes the following steps:
[0058] Step 1. Spray different concentrations of anti-plasticizer monoclonal antibody-time-resolved fluorescent microsphere dilutions on the microsphere areas of multiple test strips, place at 15°C, and treat with negative pressure for 20 minutes;
[0059] Step 2, absorb different concentrations of anti-plasticizer monoclonal antibody dilutions and goat anti-mouse IgG secondary antibody dilutions and spray them on the detection line and qua...
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