Detection method and system for stably detecting content of aflatoxin

Abstract

The invention relates to a detection method and system for stably detecting the content of aflatoxins. The detection system comprises a sealed aflatoxin detection test paper strip, an incubator and a detection reading instrument. The method enables different types of aflatoxins to be stably combined when different batches of antigens or antibodies are used for detection, wherein the inter-batch and intra-batch variation coefficients are both less than 10%. The detection system and the matched detection method are combined for use, the detection result is very stable, and the accuracy and the sensitivity are high.

Description

technical field [0001] The invention relates to the field of aflatoxin detection, in particular to a detection system and method for stably detecting the contents of various aflatoxins. Background technique [0002] Aflatoxins are toxic secondary metabolites produced by fungi. One fungus may produce multiple toxins, and multiple fungi may also produce one toxin. There are more than 1,000 kinds of aflatoxins that have been discovered in the world, and more than 20 kinds have been isolated and identified. Among them, the most harmful ones are aflatoxin AFB1, deoxynivalenol (DON), zearalenone (ZEN), and ochratoxin. Toxin A (OTA), fumonisin (FUM) and T-2 toxin. Aflatoxins widely exist in grain, oil, food and feed. According to the estimates of the Food and Agriculture Organization of the United Nations, about 25% of the world's grains are polluted by aflatoxins every year, and 2% of the crops lose their nutritional and economic value due to serious pollution. According to the...

AI Technical Summary

Problems solved by technology

However, when the traditional colloidal gold test strips are used, the package needs to be torn open, so that the test paper is exposed to the air, and the following problems occur: first, the reaction process between the test paper and the reagent is exposed to the air, not only oxidation reaction will occur, but also with the During the reaction, the sample will volatilize, which will change the volume of the sample, thereby reducing the accuracy of the detection data

[0005] Secondly, in order to promote the flow of samples in the test strips, the test strips need to be incubated during the incubation process, and the light can affect the accuracy of the test results, so light-shielding treatment is required during the incubation process, and a light-shielding seal is installed on the incubator. However, the traditional light-shielding cover cannot achieve the fastening effect when the cover is closed. Therefore, once the light-shielding cover is forced to open by a collision during the detection process, the light will shine on the incubator and affect the detection results.

[0006] Finally, the use of colloidal gold quantitative test strips to detect aflatoxin requires a reader to facilitate the user to read the value, but the traditional reader is inconvenient to disassemble and assemble. Most of them are fixed by screws. There are many screws, and it takes a lot of time to install, and it takes a lot of time to remove the screws, which is time-consuming and troublesome.

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