Thin-layer chromatography identification method of Danwei Kang capsule

A technology of thin-layer chromatography and Danweikang, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of shortening the preparation time, good separation effect and controlling the quality of medicines

Inactive Publication Date: 2021-06-04
段复华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prescription of this product consists of 10 medicinal materials, and the current drug standard only points to the qualitative id...

Method used

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  • Thin-layer chromatography identification method of Danwei Kang capsule
  • Thin-layer chromatography identification method of Danwei Kang capsule
  • Thin-layer chromatography identification method of Danwei Kang capsule

Examples

Experimental program
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Effect test

preparation example Construction

[0046] (1) Preparation of the test solution:

[0047] Take 3g of the content of "Danweikang Capsule" and add 45mL of methanol to ultrasonic treatment for 30 minutes, filter, evaporate the filtrate to dryness, add 3mL of methanol to dissolve the residue as the test solution;

[0048] (2) Thin-layer chromatographic identification of swertiamarin:

[0049] Dissolve the swertomacroside reference substance in methanol to make a 0.5 mg / mL reference substance solution; respectively draw 3 μL of the test solution and the reference solution, and place them on the same thin-layer chromatographic plate, and use chloroform-methanol -Water-formic acid solution is the developing agent for upward development, take it out, and dry it; spray the thin-layer chromatography with sulfuric acid-ethanol solution, and check it under the ultraviolet light (254nm) after drying; Blue-green fluorescent spots of the same hue appear on the corresponding position of the chromatogram of the reference substa...

Embodiment 1

[0114] Example 1: TLC Identification of Swertamarin in "Danweikang Capsules"

[0115] Take 3g of the content of "Danweikang Capsule", add 45mL of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 3mL of methanol to dissolve the residue, and use it as the test solution. Take swertamarin reference substance and dissolve it in methanol to make a 0.5mg / mL reference substance solution.

[0116] In addition, 9 medicinal materials in the prescription of "Danweikang Capsules" except Qingye Gallbladder were taken, and a negative control sample without Qingye Gallbladder was made according to the preparation method stipulated in the standard of "Danweikang Capsules", and prepared according to the above method. Become a negative control solution lacking Folium bile.

[0117] Draw 3 μL each of the test solution, reference solution, and negative control solution, respectively, and place them on the same silica gel G thin-layer chromatography pla...

Embodiment 2

[0120] Example 2: TLC Identification of Wogonin in "Danweikang Capsules"

[0121] Take 3g of the content of "Danweikang Capsule", add 45mL of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 3mL of methanol to dissolve the residue, and use it as the test solution. The wogonin reference substance was dissolved in methanol to prepare a 0.3mg / mL reference substance solution.

[0122] In addition, 9 medicinal materials of the prescription "Danweikang Capsules" except Southwest Scutellariae were taken, and a negative control sample without Southwest Scutellariae was made according to the preparation method stipulated in the standard of "Danweikang Capsules", and the lack of Southwest Scutellariae was prepared according to the above method. Negative control solution of Scutellaria baicalensis.

[0123] Draw 3 μL each of the test solution, reference solution, and negative control solution, respectively, and place them on the same silica gel...

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Abstract

The invention relates to the field of pharmaceutical analysis, and provides a thin-layer chromatography identification method of a Danwei Kang capsule, which is characterized in that swertiamarin, wogonin, naringin, neohesperidin, alisol B, 23-acetyl alisol B and pachymic acid are added as reference substances; identification objects respectively point to seven thin-layer chromatography qualitative identification indexes of swertia mileensis, southwest scutellaria baicalensis, fructus aurantii, rhizoma alismatis and poria cocos which are main medicinal materials of a Danweishang capsule formula. Compared with the current drug standard of the Danwei Kang capsule, the method overcomes the technical defect that the current standard of the Danwei Kang capsule has few quality control points for identifying the medicinal materials of the prescription, and the provided identification item index is more comprehensive, strong in specificity, simple and rapid to operate, visual and clear in judgment and objective and reliable in conclusion, the invention provides a technical method for perfecting and improving the quality standard of the national medicine Danshenkang capsule and better controlling the quality of the medicine.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a TLC identification method for Danweikang capsules. Background technique [0002] The prescription of ethnic medicine "Danweikang Capsule" comes from Mr. Duan Hongguang, a famous ethnic medicine expert in Baoyuantang, Yunnan. It has been clinically used for more than 40 years to treat rib pain, jaundice, bile reflux gastritis and cholecystitis caused by liver and gallbladder damp heat. curative effect. Its prescription consists of 10 medicinal materials including Qingyedan, Citrus aurantium, Southwest Scutellaria baicalensis, Bamboo-leaf Bupleurum, Paeoniae Alismatis, Alisma, Poria, Yin Chen, Pale Bamboo Leaf, and Rush. Among them, Qingyedan, Southwest Scutellaria, Bamboo Leaf Bupleurum, etc. are characteristic ethnic medicinal materials in Yunnan. The prescription composition and preparation method of this product are protected by the invention patent "medic...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94Y02A50/30
Inventor 段复华饶无忌杨洪英
Owner 段复华
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