Negative and positive classification model establishment method and device, equipment and computer storage medium

Abstract

The invention provides a negative and positive classification model establishment method and device, equipment and a storage medium, and is applied to a dPCR system of which the heat dissipation efficiency is lower than a preset value, and the method comprises: aiming at a single dPCR amplification reaction, respectively selecting a first number of negative samples and positive samples; respectively selecting a second number of negative samples and positive samples as training samples, inputting the training samples into a preset SVM training model in an out-of-order manner, and solving a first hyperplane model meeting a preset requirement; and respectively taking a third number of negative samples and positive samples as test samples, sequentially inputting the test samples into the first hyperplane model, and determining the first hyperplane model as a negative and positive classification model of the dPCR system when the accuracy of the category of the output test samples reaches a set threshold value, wherein the sum of the second number and the third number is the first number, and the second number and the third number belong to the first number. According to the scheme, the classification accuracy is high, the accuracy of dPCR quantification can be effectively guaranteed, and the dPCR amplification process can be tracked.

Description

technical field [0001] The invention relates to the technical field of establishing negative-positive classification models for test samples, and in particular to a method, device, equipment and computer storage medium for establishing negative-positive classification models. Background technique [0002] Digital PCR (Digital Polymerase Chain Reaction, dPCR) is a high-sensitivity, high-accuracy nucleic acid absolute quantification technology. It has attracted more and more attention because it can achieve absolute quantification of target nucleic acids without any correction, and has been widely used in quantitative analysis of viruses, rare gene screening, copy number variation research, pathogen detection, prenatal gene diagnosis etc. The dPCR reaction is mostly carried out in microfluidic chips or oil droplets, and the fluorescence intensity at the end of the amplification is used to distinguish negative and positive. The microfluidic chip can quickly and accurately div...

AI Technical Summary

Problems solved by technology

However, DNA amplification efficiency and result accuracy are easily affected by slight changes in reaction conditions

In practical applications, there are two problems in the PCR reaction process: first, the false negative problem caused by the low amplification efficiency of the reaction system in different microwells; second, non-specific probe hybridization, microwell evaporation or dPCR brushing False positive problems caused by irregularities

It is difficult to distinguish false positive points and false negative points existing in the amplification process, which affects the accuracy of digital PCR quantification

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