Kit for detecting multiple steroid hormones in biological fluid and use method of kit

A technology for steroid hormones and biological fluids, applied in the fields of analytical chemistry and clinical testing, can solve the problems of low detection sensitivity, long detection time, and low detection efficiency, and achieve the effects of eliminating low sensitivity, high accuracy and easy operation.

Pending Publication Date: 2021-06-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a test kit for detecting multiple steroid hormones in biological fluids and a method for using the same, which can solve the problems of long detection time, low detection efficiency and low detection sensitivity of existing steroid hormone detection methods

Method used

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  • Kit for detecting multiple steroid hormones in biological fluid and use method of kit
  • Kit for detecting multiple steroid hormones in biological fluid and use method of kit
  • Kit for detecting multiple steroid hormones in biological fluid and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] The present embodiment adopts kit of the present invention and using method thereof to treat 9 kinds of steroid hormones (cortisol, cortisone, 11-deoxycortisol, corticosterone, dehydroepiandrosterone, testosterone, 17-hydroxyprogesterone, androsne diketone and progesterone) were verified for accuracy.

[0114] 1. Kit and its composition

[0115] (1) Standard product working solution: prepare cortisol, cortisone, 11-deoxycortisol, corticosterone, dehydroepiandrosterone, testosterone, 17-hydroxyprogesterone, androstenedione and progesterone respectively with methanol Stock solution 1mg / mL, then dilute the above stock solution with 50% methanol solution into a standard working solution, a total of 5 concentration points, the concentration of steroid hormones at each point is as follows:

[0116] Cortisol 10ng / mL, 20ng / mL, 100ng / mL, 500ng / mL, 2500ng / mL;

[0117] Cortisone 2ng / mL, 4ng / mL, 20ng / mL, 100ng / mL, 500ng / mL;

[0118] 11-deoxycortisol 0.1ng / mL, 0.2ng / mL, 1ng / mL, 5...

Embodiment 2

[0178] In this embodiment, the kit and its usage method are the same as those in Embodiment 1, the difference is that this embodiment includes a pretreatment method for biological fluid samples.

[0179] In this embodiment, the contents of 9 steroid hormones in 13 plasma samples were detected.

[0180] Biological fluid samples were collected at the Seventh People's Hospital of Dalian, and all volunteers collected blood on an empty stomach between 7:00 and 8:00 in the morning. Whole blood was collected in vacuum blood collection tubes containing anticoagulants, centrifuged at 3000rpm for ten minutes, and the upper layer of plasma was collected and stored at -80°C for later use.

[0181] Pretreatment method for biological fluid samples: add 20 μL internal standard working solution to 200 μL plasma sample, add 1 mL liquid-liquid extractant, vortex for 3 minutes, centrifuge at 15000 rpm for 10 minutes, take the supernatant and dry it with nitrogen at 40 °C, add 90 50 μL of % acet...

Embodiment 3

[0186] In this embodiment, the kit and its usage method are the same as in Embodiment 2, except that the biological fluid sample is different, and the biological fluid sample in this embodiment is serum.

[0187] In this embodiment, the contents of 9 steroid hormones in 13 serum samples were detected.

[0188] Samples were collected at Dalian Central Hospital, and all volunteers collected blood on an empty stomach between 7:00 am and 8:00 am. Whole blood was collected in vacuum blood collection tubes without anticoagulant, centrifuged at 3000rpm for ten minutes after standing for coagulation, and the upper serum layer was collected and stored at -80°C for later use.

[0189] See Table 13 for the contents of 9 steroid hormones in the serum samples of 13 cases.

[0190] Table 1413 Serum Samples of 9 Steroid Hormone Contents

[0191]

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Abstract

The invention discloses a kit for detecting multiple steroid hormones in biological fluid and a use method of the kit, belongs to the technical field of analytical chemistry and clinical examination, and aims to solve the problems of long detection time, low detection efficiency and low detection sensitivity of an existing steroid hormone detection method. The kit for detecting the multiple steroid hormones in the biological fluid comprises a standard substance/standard substance working solution, a quality control substance/quality control substance working solution, an internal standard substance/internal standard substance working solution, a matrix, a liquid-liquid extraction solution and an eluent. According to the kit for detecting the multiple steroid hormones in the biological fluid and the use method of the kit of the present invention, the steroid hormones in the biological fluid sample are directly detected according to the inherent physicochemical property mass/charge ratio of an analyte by using the liquid chromatography-tandem mass spectrometry technology, and the non-derivatization method is adopted, such that the operation is simple, the contents of the plurality of steroid hormones in the biological fluid sample can be simultaneously detected, the detection time is substantially shortened, and the clinical detection requirements on the steroid hormones can be met.

Description

technical field [0001] The invention relates to the technical fields of analytical chemistry and clinical testing, in particular to a kit for detecting multiple steroid hormones in biological fluids and a method for using the same. Background technique [0002] Steroid hormones are fat-soluble small molecules that are catalyzed by a series of enzymes from cholesterol, and have important functions such as maintaining metabolism and regulating sexual function. Under the catalysis of various enzymes in the human body, steroid hormones will be metabolized with each other, and will be continuously produced or consumed, thereby maintaining a dynamic balance. Therefore, it is necessary to accurately detect the concentration of each steroid hormone clinically, and to understand the direct metabolic relationship between its upstream and downstream products. This can provide assistance for the diagnosis of endocrine system diseases, male / female reproductive system diseases, tumors, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/045G01N2030/8822G01N2030/062
Inventor 张晓哲刘欣欣刘丹程孟春赵楠
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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