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A method and application of preparing concentrated protein powder and xylo-oligosaccharides by using mulberry leaves

A technology of concentrated protein powder and hypoglycemia, which is used in the field of feed additives, can solve problems such as no concentrated protein powder and hypoglycerum, and achieve enhancement of animal immunity, methods, and saving drying costs. Effect

Active Publication Date: 2022-05-17
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on the preparation of concentrated protein powder and xylooligosaccharides by using the leaves of the tree and applying them to the field of animal breeding.

Method used

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  • A method and application of preparing concentrated protein powder and xylo-oligosaccharides by using mulberry leaves
  • A method and application of preparing concentrated protein powder and xylo-oligosaccharides by using mulberry leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of Bacillus subtilis PL83 fermentation broth:

[0032] A, get 2ml bacterial strain A (Bacillus subtilis PL83), live bacteria concentration is 10 10 CFU / ml;

[0033] B. Inoculate in 100ml LB medium (composition: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000ml, pH value 7.2) and carry out shake flask fermentation culture, cultivate for 24 hours.

[0034] C. The fermentation temperature is 28 or 30 or 32 or 35 or 37°C, the pH value is 7.2, the rotation speed is 200-300r / min, and the fermentation time is 24 or 28 or 32 or 36h. The shake flask fermentation medium is LB medium, the ingredients are: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000ml, and the pH value is 7.2.

[0035] D. After the shake flask fermentation is completed, carry out the expansion culture of the fermenter, take 100ml shake flask fermentation seed liquid and inoculate it into a 10L fermenter, the liquid volume is 6L, the fermentation ...

Embodiment 2

[0047] The concentrated protein powder and xylo-oligosaccharide prepared in Example 1 can be used in the preparation of animal feed: add 1-10% of the concentrated protein powder of mulberry leaf to the full-price compound feed, mix well, and feed animals. Add 0.1-5% of xylo-oligosaccharides from leaves to the full-price compound feed, mix thoroughly, and feed animals.

Embodiment 3

[0048] Embodiment 3: Concentrated leaf protein powder and xylo-oligosaccharide pig raising test

[0049] 21-day-old weaned piglets with similar individuals were randomly selected to form a control group and an experimental group at random, with 30 pigs in each group, and each group was divided into 3 pens with 10 pigs in each pen. The control group was fed with the basal diet, and the formula was shown in Table 1:

[0050] Table 1

[0051]

[0052]

[0053] Premix * Contains per kg: 10.00mg copper; 100.00mg iron; 0.30mg sodium; 100.00mg zinc; 10.00mg magnesium; 386.0IU vitamin D3; 3 086.0IU vitamin A; 15.4IU vitamin E; ; 15.40mg D-pantothenic acid; 23.00mg niacin; 77.00mg choline; and 15.4μg vitamin B12.

[0054] The test group is two groups, one group is to add 3% (mass ratio) structure leaf protein concentrate on the basis of constant formula, and one group is to add 3% (mass ratio) structure leaf xylo-oligosaccharide on the basis of constant formula And get.

[0...

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Abstract

The invention relates to a method and application for preparing concentrated protein powder and xylo-oligosaccharides by using the leaves of the tree leaves, and the method is as follows: crushing the leaves of the tree leaves and extruding to obtain an extrusion liquid rich in protein and containing cellulose and lignin , Hemicellulose-rich residue. Spray-dry the extruded liquid to obtain concentrated protein powder of mulberry leaves; carry out microbial fermentation, homogenization and enzymolysis on the residue to obtain mulberry leaf xylo-oligosaccharides rich in xylo-oligosaccharides, which can be used in animal breeding and have good Its growth-promoting and immune-enhancing effects have excellent economic value.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to a method and application for preparing concentrated protein powder and xylo-oligosaccharides by utilizing the leaves of mulberry leaves. Background technique [0002] The leaves of mulberry tree are rich in protein, the crude protein content in its dry matter can reach 26-32%, the amino acid content is 4.5 times that of rice, 2.5 times that of corn, 1.8 times that of soybean, and the content of vitamins and trace elements is the largest. Several times of most fruits and vegetables have always been used as pig feed by common people. However, there are also some problems in the breeding process, such as the palatability of the leaf powder. Due to its high fiber content, the palatability of the leaves is poor, and adding them to the feed causes the animal's feed intake to decrease. [0003] Xylooligosaccharides, also known as xylooligosaccharides, are important f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04A23J1/00A23K20/163A23K20/147A23K50/30A23K50/75A23K50/10A23K50/50A23K50/70C12P19/12C12P19/00
CPCA23J1/006A23K20/163A23K20/147A23K50/30A23K50/75A23K50/10A23K50/50A23K50/70C12P19/12C12P19/04C12P19/00C12P19/14C07K1/14Y02P60/87
Inventor 王升平曾黎明陈勇刘志强赵君存
Owner HUNAN INST OF MICROBIOLOGY
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