Method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis

A peony seed oil, ultrasonic-assisted technology, applied in the direction of fat production, fat oil/fat production, fat oil/fat refining, etc., can solve the problem that the oil cannot completely flow out, and achieve strong anti-oxidation, pure flavor, and unsaturated fatty acid content high effect

Inactive Publication Date: 2021-06-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single enzyme has limited damage to the cell structure and oil complex of peony seeds, making the oil unable to flow out completely

Method used

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  • Method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis
  • Method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis
  • Method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0024] Pulverize peony seeds in a pulverizer, pass through a 60-mesh sieve to obtain the undersize material for use; mix the undersize material with deionized water in a ratio of 1:5 and stir evenly to form a semi-solid mixed solution, and mix the mixed solution Under the conditions of ultrasonic power of 300W and ultrasonic temperature of 45°C, perform ultrasonic treatment for 55 minutes; adjust the pH value of the mixture after ultrasonic treatment to 5.0, add 0.5% (v / w) cellulase for enzymatic hydrolysis for 1 hour, and inactivate the enzyme at 90°C 10min, then adjust the pH of the mixed solution to 9.0, add 1.5% (v / w) alkaline protease protex 6L for enzymolysis for 2h, and inactivate the enzyme at 90°C for 10min; cool the enzymolyzate after inactivation to 4°C and perform high-speed centrifugation , to obtain free oil, emulsion, hydrolyzate, and residue; the emulsion was frozen and demulsified at -20°C for 24 hours, and after thawing at 40°C, it was centrifuged at 10,000 g ...

Embodiment example 2

[0026] Pulverize peony seeds in a pulverizer, pass through a 60-mesh sieve to obtain the undersize material for use; mix the undersize material with deionized water in a ratio of 1:5 and stir evenly to form a semi-solid mixed solution, and mix the mixed solution Under the conditions of ultrasonic power of 400W and ultrasonic temperature of 40°C, perform ultrasonic treatment for 45 minutes; adjust the pH value of the mixture after ultrasonic treatment to 5.0, add 0.5% (v / w) cellulase for enzymatic hydrolysis for 1 hour, and inactivate the enzyme at 90°C 10min, then adjust the pH of the mixed solution to 9.0, add 1.5% (v / w) alkaline protease protex 6L for enzymolysis for 2h, and inactivate the enzyme at 90°C for 10min; cool the enzymolyzate after inactivation to 4°C and perform high-speed centrifugation , to obtain free oil, emulsion, hydrolyzate, and residue; the emulsion was frozen and demulsified at -20°C for 24 hours, and after thawing at 40°C, it was centrifuged at 10,000 g ...

Embodiment example 3

[0028] Pulverize peony seeds in a pulverizer, pass through a 60-mesh sieve to obtain the undersize material for use; mix the undersize material with deionized water in a ratio of 1:5 and stir evenly to form a semi-solid mixed solution, and mix the mixed solution Under the conditions of ultrasonic power of 500W and ultrasonic temperature of 30°C, perform ultrasonic treatment for 35 minutes; adjust the pH value of the mixture after ultrasonic treatment to 5.0, add 0.5% (v / w) cellulase for enzymatic hydrolysis for 1 hour, and inactivate the enzyme at 90°C 10min, then adjust the pH of the mixed solution to 9.0, add 1.5% (v / w) alkaline protease protex 6L for enzymolysis for 2h, and inactivate the enzyme at 90°C for 10min; cool the enzymolyzate after inactivation to 4°C and perform high-speed centrifugation , to obtain free oil, emulsion, hydrolyzate, and residue; the emulsion was frozen and demulsified at -20°C for 24 hours, and after thawing at 40°C, it was centrifuged at 10,000 g ...

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Abstract

The invention discloses a method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis, which belongs to the field of food processing. The method comprises the following steps of (1) crushing peony seeds, and then sieving to obtain peony seed powder, (2) adding water into the peony seed powder, mixing to form a semi-solid mixed solution, and carrying out ultrasonic treatment on the semi-solid mixed solution, (3) adding a compound enzyme into the mixed solution subjected to ultrasonic treatment for enzymolysis, wherein the compound enzyme comprises cellulase and alkaline protease protex6L, (4) cooling the enzymatic hydrolysate after enzyme deactivation, and performing high-speed centrifugation to obtain free oil, emulsion, hydrolysate and residues, and (5) freezing and demulsifying the emulsion, then thawing and centrifuging to take supernatant oil, and combining the supernatant oil with free oil to obtain peony seed oil. The ultrasonic-assisted biological enzymolysis extraction technology adopted by the invention is a novel peony seed oil extraction method, and the method has the advantages of high peony seed oil extraction rate, strong oxidation resistance, high unsaturated fatty acid content, high total phenol content, light color and pure flavor.

Description

technical field [0001] The invention belongs to food processing technology, and mainly relates to a method for extracting peony seed oil through ultrasonic-assisted biological enzymolysis. Background technique [0002] Peony seed oil is currently found to be the most suitable oil for the nutritional needs of the human body. It has a reasonable composition structure, comprehensive nutritional value, rich in unsaturated fatty acids needed by humans, and also has anti-inflammatory, liver protection, hypoglycemic and immune regulation effects. . The key to the extraction of peony seed oil is the destruction of cell walls, lipid bodies and protein bodies. [0003] Enzymatic oil extraction (EAEP) technology is an emerging and environmentally friendly vegetable oil extraction technology. It uses biological enzymes to degrade macromolecular substances (lipoproteins, lipopolysaccharides, etc.) in oils on the basis of physical crushing, so that water and non-oil The increased affini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C11B3/00C11B3/16
CPCC11B1/025C11B3/001C11B3/00C11B3/16C11B1/00
Inventor 江连洲连子腾廖培龙彭新辉佟晓红
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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