Target molecule detection method based on molecularly imprinted polymer

Abstract

The invention discloses a molecularly imprinted polymer-based target molecule detection method, which comprises the following steps of: firstly, loading a specific molecularly imprinted polymer into a solid-phase extraction column by taking the specific molecularly imprinted polymer as a recognition unit; connecting the upper part of the solid-phase extraction column with a container filled with water, and loading the lower part of the solid-phase extraction column on a solid-phase extraction instrument; connecting the solid-phase extraction column with a vacuum pump for vacuumizing so that water at the upper part quickly flows; and recording the time when the water completely passes through the solid-phase extraction column by using a timer, and converting molecular detection into time detection to realize the detection of specific target molecules. The method can realize the detection of different target molecules, is simple and convenient, does not need expensive instruments and professional operation, and can be applied to the rapid detection of trace hydroxyl polychlorinated biphenyl in a water sample.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a molecularly imprinted polymer-based target molecule detection method. Background technique [0002] Point of care test (POCT) is a rapid, convenient and intelligent detection technology, which is widely used in home health care, mobile medical care, field environment and battlefield medical care. At present, on-site testing mostly uses blood glucose meters, thermometers, microfluidic chips, etc. to read data, but the above tools all need to convert the system. Therefore, there is an important practical demand for a point-of-care testing technology with simple principle, convenient operation, good selectivity and high accuracy. [0003] Molecular imprinting polymers (molecular imprinting polymers, MIPs) are polymers synthesized by molecular imprinting technology that can specifically recognize and selectively adsorb specific target molecules (template molecules) an...

AI Technical Summary

Problems solved by technology

If the molecularly imprinted polymer is filled into the microchannel, the permeability of the channel will decrease after the target molecule is adsorbed, and if a certain amount of liquid is injected into it, the time required for the liquid to completely flow out will increase.

In the detection process of target molecules, hydroxy polychlorinated biphenyls (OH-PCBs) is a common secondary pollutant, which is produced by the oxidation of polychlorinated biphenyls (PCBs) through biological or abiotic methods. In the prior art, the commonly used detection methods for hydroxy-PCBs are instrumental methods (GC-MS, LC-MS, MS, etc.), the pretreatment process is complex and time-consuming, and it is difficult to carry out hydroxy-PCBs in the water environment. Efficient Extraction and Detection

Images