Micro-droplet biological analysis reagent card and analysis system

A biological analysis and analysis system technology, applied in the field of biological detection and analysis, can solve the problems of not meeting the precision required by the micro-droplet analysis technology, not taking into account the fluid dynamics in the oscillation process, and insufficient reaction between samples and reagents, etc. The detection rate and accuracy, the mixing effect is good, and the work reliability is improved

Inactive Publication Date: 2021-06-18
吴庆金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of specific implementation, it was found that it did not take into account the fluid dynamics in the oscillation process, so that the results of different batches of tests under the same test conditions were quite different, and the precision required by the micro-droplet analysis technology was not met. And because the bottom surface of the series microcell and the slit is a smooth and thin-walled structure, there are often cases where the reaction between the sample and the reagent is insufficient.

Method used

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  • Micro-droplet biological analysis reagent card and analysis system
  • Micro-droplet biological analysis reagent card and analysis system
  • Micro-droplet biological analysis reagent card and analysis system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as Figure 1 to Figure 9 Shown is a first embodiment of a micro-droplet biological analysis reagent card, including a curved substrate 101, five curved grooves 102 and four curved channels 103 arranged on the curved substrate 101, adjacent arcs The arc-shaped grooves 102 communicate with each other through an arc-shaped channel 103; the inner wall of the arc-shaped groove 102 is provided with several protrusions 104.

[0035] Of course, the number of arc-shaped grooves 102 and arc-shaped passages 103 is only a reference implementation, which is to describe the structural principle of this solution more clearly, and should not be understood as a limitation to this solution.

[0036] Such as figure 2 , image 3As shown, the side of each arc-shaped groove 102 away from the center of the circle is the outer wall 1025, the side close to the center of the circle is the inner wall 1024, and the part connecting the inner side wall 1024 and the outer side wall 1025 is th...

Embodiment 2

[0056] Such as Figure 10 Shown is a second embodiment of a micro-droplet biological analysis reagent card. The difference between this embodiment and the first embodiment is only that the volumes of the reaction tank 1021, the washing tank 1022, and the detection tank 1023 in this embodiment, The bottom areas are all different, wherein the width of the reaction tank 1021 is 2mm, the depth is 8mm, and the bottom area is 30mm 2 ; The width of the washing tank 1022 is 3mm, the depth is 8mm, and the bottom surface area is 40mm 2 ; The detection groove 1023 has a width of 4mm, a depth of 8mm, and a bottom area of ​​80mm 2 . In this way, the reaction tank 1021 as a whole will be narrower, which is convenient for detecting the combination of samples and reagents, and the washing tank 1022 is wider, so that after the magnetic particles enter the washing tank 1022 from the reaction tank 1021, they can be fully wrapped by the washing solution for washing. 1023 is the widest, can coo...

Embodiment 3

[0058] The difference between this embodiment and the second embodiment is that in this embodiment, the width of the arc channel 103 is 0.5 mm, the width of the reaction tank 1021 is 1 mm, the depth is 3 mm, and the area of ​​the bottom surface is 10 mm. 2 ; The width of the washing tank 1022 is 1mm, the depth is 3mm, and the bottom surface area is 10mm 2 ; The detection groove 1023 has a width of 1mm, a depth of 3mm, and a bottom area of ​​10mm 2 .

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Abstract

The invention belongs to the technical field of biological detection and analysis, and particularly relates to a micro-droplet biological analysis reagent card and an analysis system. The micro-droplet biological analysis reagent card comprises an arc-shaped base plate, a plurality of arc-shaped grooves formed in the arc-shaped base plate, and arc-shaped grooves, wherein the adjacent arc-shaped grooves are communicated through arc-shaped channels; and a plurality of protrusions are arranged on the inner walls of the arc-shaped grooves. The arc-shaped reagent card can adapt to rotary oscillation, so that the uniform mixing effect is better, the arc-shaped reagent card can be directly driven by a driving motor, excessive transmission parts such as screws are not needed, the manufacturing cost of the device is reduced, and the working reliability of the device is improved; in addition, the protrusions are correspondingly arranged on the inner walls of the arc-shaped grooves, so that the mixing speed is higher, and the effect is better.

Description

technical field [0001] The invention belongs to the technical field of biological detection and analysis, and more specifically relates to a micro-droplet biological analysis reagent card and an analysis system. Background technique [0002] The current clinical biomedical detection technology is changing with each passing day. Large-scale instruments have various advantages such as stable detection, high throughput, accuracy, high sensitivity, and flexible reagent detection. However, they are large in size and high in cost. Scenario use of the machine. [0003] In view of this, the droplet analysis technology has gradually developed and matured. It uses magnetic force to act on a small volume of liquid (generally an oil phase) wrapped with magnetic particles, and then manipulates it to move, and realizes detection steps such as reaction, washing, and detection. The technology is usually implemented on an analysis chip, which can make the detection equipment smaller. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N35/02G01N35/04B01L3/00
CPCB01L3/5027G01N35/00G01N35/025G01N35/04G01N2035/00148G01N2035/00158G01N2035/00465
Inventor 吴庆金吴海林
Owner 吴庆金
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