Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray

Abstract

The disclosure describes apparatus and methods for multiplexed amplification and detection of nucleic acid targets in a sample. Embodiments of the present disclosure include a mechanical system configured to provide loading, vertical positioning and clamping of a chip; a thermal control system configured to maintain distinct temperatures of the chip, and an optical fluorescence imaging system.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 62 / 731,495, filed September 14, 2018, the entire contents of which are incorporated herein by reference. technical field [0003] The present disclosure relates to devices and methods for multiplex amplification and detection of DNA using convective heating and label-free microarrays. Background technique [0004] This invention was made with government support under Grant No. R01CA203964 awarded by the National Institutes of Health and the National Cancer Institute. The government has certain rights in this invention. [0005] related technology [0006] Government agencies, hospitals and doctor's offices, as well as private consumers have strong market demand for rapid and multiplex DNA diagnostics. Current commercial qPCR systems are bulky and expensive, and are limited to simultaneous detection of 4-6 DNA markers of interest (4-6 ...

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Problems solved by technology

However, the reported convective PCR instruments do not integrate microarrays for high-repeat DNA analysis, therefore, no reported convective-PCR instruments use cameras with a pixel resolution of less than 100 μm to acquire images of fluorescent spots, which is necessary to achieve high-repeat reads. necessary

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