Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray

A multiplex amplification, heating block technology, applied in the field of devices and methods for multiplex amplification and detection of DNA using convection heating and label-free microarrays

Pending Publication Date: 2021-06-18
RICE UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported convective PCR instruments do not integrate microarrays for high-repeat DNA analysis, therefore, no reported convective-PCR instruments use cameras with a pixel resolution of less than 100 μm to acquire images of fluorescent spots, which is necessary to achieve high-repeat reads. necessary

Method used

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  • Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray
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  • Apparatus and methods for multiplexed amplification and detection of DNA using convectional heating and label-free microarray

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Embodiment Construction

[0040] Exemplary embodiments of the present disclosure include three main components: (1) chip: annular reaction chamber for reliable convective PCR without active cooling or fluidics; (2) assay and readout: using a single fluorescent channel via Spatial separation of probes in microarrays for simultaneous detection and analysis of 50 or more DNA targets in a closed-tube format; and (3) device: a portable and affordable instrument that enables multiplex amplification and real-time readout out.

[0041] The principle of convective PCR is that an aqueous solution can be controllably circulated due to a temperature-induced density difference (Rayleigh-Benard convection). In short, hotter solutions are less dense and cooler solutions are denser, so gravity drives the circulation of differentially heated solutions. Since no active cooling or fluidic components are required, the size and weight of a convective PCR instrument will be significantly lower than conventional PCR using t...

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Abstract

The disclosure describes apparatus and methods for multiplexed amplification and detection of nucleic acid targets in a sample. Embodiments of the present disclosure include a mechanical system configured to provide loading, vertical positioning and clamping of a chip; a thermal control system configured to maintain distinct temperatures of the chip, and an optical fluorescence imaging system.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 62 / 731,495, filed September 14, 2018, the entire contents of which are incorporated herein by reference. technical field [0003] The present disclosure relates to devices and methods for multiplex amplification and detection of DNA using convective heating and label-free microarrays. Background technique [0004] This invention was made with government support under Grant No. R01CA203964 awarded by the National Institutes of Health and the National Cancer Institute. The government has certain rights in this invention. [0005] related technology [0006] Government agencies, hospitals and doctor's offices, as well as private consumers have strong market demand for rapid and multiplex DNA diagnostics. Current commercial qPCR systems are bulky and expensive, and are limited to simultaneous detection of 4-6 DNA markers of interest (4-6 ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L7/00G01N21/00C12Q1/00G01N21/62G01N21/75
CPCB01L7/525B01L3/502715B01L9/527B01L2200/147B01L2300/1805G01N21/6458B01L2400/0445B01L2300/0816B01L2300/0822B01L2300/088B01L2200/04B01L2200/0663C12Q2537/143B01L9/52B01L2200/0689B01L2300/027C12Q1/686
Inventor 大卫·于·张德米特里·A·霍达科夫张雪萌
Owner RICE UNIV
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