Fluorescent magnetic resonance bimodal imaging nano sensing material as well as preparation method and application thereof

A dual-mode imaging and sensing material technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of poor sensitivity, spatial resolution and poor tissue penetration, achieve short relaxation time, improve biological Compatibility, effect of reducing cytotoxicity

Pending Publication Date: 2021-06-22
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, individual imaging modes have certain limitations
For example, despite the high three-dimensional spatial resolution and deep tissue penetration of magnetic resonance imaging, its sensitivity is poor
Fluorescence imaging has single-cell sensitivity and subcellular resolution, but suffers from poor spatial resolution and tissue penetration

Method used

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  • Fluorescent magnetic resonance bimodal imaging nano sensing material as well as preparation method and application thereof
  • Fluorescent magnetic resonance bimodal imaging nano sensing material as well as preparation method and application thereof
  • Fluorescent magnetic resonance bimodal imaging nano sensing material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1ml 0.01mol / L TbCl 3 Ethanol solution and 1ml of 0.01mol / L GdCl 3 The ethanol solution was stirred and mixed, and 2ml of 0.03mol / L acac ethanol solution was added, and a magnetic stirrer was used at a rotating speed of 850rpm to stir at room temperature for 0.5 hours. Use dilute ammonia to adjust pH to 7, and then add 2ml of 0.01mol / L phen ethanol solution, The mixture was stirred at room temperature for 1.5 hours to obtain a rare earth gadolinium terbium binuclear complex solution.

[0030] Take 2ml of 10mg / ml sodium hyaluronate solution (prepared by 0.1mol / L, pH=5 acetic acid-sodium acetate buffer solution as solvent), add it to the rare earth gadolinium terbium binuclear complex solution obtained above, Stir at room temperature for 2 hours, then take 4 ml of 10 mg / ml chitosan solution (prepared from 0.1 mol / L, pH=5 acetic acid-sodium acetate buffer solution as solvent), add it to the solution, and stir at room temperature for 2.5 hours , obtain rare earth gadoliniu...

Embodiment 2

[0034] 1ml 0.01mol / L TbCl 3 Ethanol solution and 1ml of 0.01mol / L GdCl 3 The ethanol solution was stirred and mixed, and 2ml of 0.03mol / L acac ethanol solution was added, and a magnetic stirrer was used at a rotating speed of 850rpm to stir at room temperature for 0.5 hours. Use dilute ammonia to adjust pH to 7, and then add 2ml of 0.01mol / L phen ethanol solution, The mixture was stirred at room temperature for 1.5 hours to obtain a rare earth gadolinium terbium binuclear complex solution.

[0035] 1ml 0.01mol / L TbCl 3 Ethanol solution, add 1ml of 0.03mol / L acac ethanol solution, use a magnetic stirrer at a rotating speed of 850rpm, stir at room temperature for 0.5 hours, use dilute ammonia to adjust pH to 7, then add 1ml of 0.01mol / L phen ethanol solution, stir at room temperature After 1.5 hours, 3 ml of ethanol was added for dilution to obtain a rare earth terbium complex solution.

[0036] The fluorescence test was carried out on the rare earth gadolinium terbium binucl...

Embodiment 3

[0038] 1ml 0.01mol / L TbCl 3 Distilled aqueous solution and 1ml of 0.01mol / L GdCl 3 The distilled aqueous solution was stirred and mixed, the tta distilled aqueous solution of 2ml 0.03mol / L was added, and a magnetic stirrer was used under rotating speed 850rpm, and normal temperature was stirred for 0.5 hour, and the dilute ammonia water was used to adjust pH to 7, and then the phen distilled water solution of 2ml 0.01mol / L was added, The mixture was stirred at room temperature for 1.5 hours to obtain a rare earth gadolinium terbium binuclear complex solution.

[0039]Take 2ml of 10mg / ml sodium hyaluronate solution (prepared by 0.1mol / L, pH=5 acetic acid-sodium acetate buffer solution as solvent), add it to the rare earth gadolinium terbium binuclear complex solution obtained above, Stir at room temperature for 2 hours, then take 4 ml of 10 mg / ml chitosan solution (prepared from 0.1 mol / L, pH=5 acetic acid-sodium acetate buffer solution as solvent), add it to the solution, and...

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Abstract

The invention discloses a fluorescent magnetic resonance bimodal imaging nano sensing material as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing a rare earth gadolinium terbium binuclear complex: mixing gadolinium chloride with a terbium chloride solution, dropwise adding a first organic ligand solution into the solution, conducting stirring at normal temperature by a magnetic stirrer, adjusting the pH value of the reaction solution to 6-8 by using dilute ammonia water, adding a second organic ligand solution, and conducting stirring to prepare a rare earth gadolinium terbium binuclear complex; and preparing a rare earth gadolinium terbium natural biological polyelectrolyte hybrid material: adding a negatively charged natural biological polyelectrolyte solution into the rare earth gadolinium terbium binuclear complex, conducting stirring at normal temperature by a magnetic stirrer, adding a positively charged natural biological polyelectrolyte solution, and conducting stirring at normal temperature to prepare the rare earth gadolinium terbium natural biological polyelectrolyte hybrid material. The fluorescent magnetic resonance bimodal imaging nano sensing material is used for fluorescence / magnetic resonance bimodal imaging, not only has single cell sensitivity and subcellular resolution, but also has better spatial resolution and tissue penetrability, and provides more information in clinical research.

Description

technical field [0001] The invention relates to a rare earth gadolinium-terbium hybrid material and a preparation method thereof, in particular to a fluorescence magnetic resonance dual-mode imaging nano-sensing material, a preparation method and application thereof. Background technique [0002] Molecular imaging technology is one of the important methods of clinical diagnosis, including magnetic resonance imaging (MRI), X-ray computed tomography (CT), positron emission tomography (PET) and fluorescence imaging (FI), etc. has its own characteristics. However, a single imaging mode has certain limitations. For example, although magnetic resonance imaging has high three-dimensional spatial resolution and deep tissue penetration, its sensitivity is poor. Fluorescence imaging has single-cell sensitivity and subcellular resolution, but its spatial resolution and tissue penetration are poor. Therefore, we combine various imaging techniques to take full advantage of their respe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/12A61K49/18B82Y5/00B82Y15/00
CPCA61K49/0002A61K49/0019A61K49/0054A61K49/0093A61K49/126A61K49/1863B82Y5/00B82Y15/00
Inventor 唐建国李泰森王新芝王瑶刘瑾王彦欣王薇黄林军李龙龙李淑鑫劳伦斯·巴菲奥
Owner QINGDAO UNIV
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