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Method for detecting pesticide carbaryl based on nanogold etching

An etching detection, nano-gold technology, applied in measurement devices, material analysis, instruments and other directions by observing the impact on chemical indicators, can solve the problems of high professional technical requirements, expensive instruments, unstable enzymes, etc., to achieve identification and The effect of strong quantitative ability, rapid response and efficient measurement

Active Publication Date: 2021-06-25
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the advantages of high sensitivity and low cross-interference, but at the same time, they have disadvantages such as time-consuming, expensive instruments, high professional technical requirements, and unstable enzymes.

Method used

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  • Method for detecting pesticide carbaryl based on nanogold etching
  • Method for detecting pesticide carbaryl based on nanogold etching
  • Method for detecting pesticide carbaryl based on nanogold etching

Examples

Experimental program
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Effect test

Embodiment 1

[0061] A method for the visual detection of the pesticide carbaryl based on nano-gold etching, the schematic diagram of the detection principle is shown in image 3 , including the following steps:

[0062] The carbaryl concentration was 5×10 -4 -5×10 -5 mol / L of 50 μL water matrix and 80 μL of the obtained nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution I was added to a 96-well plate containing 150 μL of the resulting biconical gold nanometer solution (0.9575 g / ml) and 20 μL of hydrogen peroxide (3mol / L) mixed solution, and supplemented with water Until the volume of the obtained solution system is 400 μL, react at 40°C for 45 minutes, use a smart phone to take pictures of the color changes of the solution in the 96-well plate before and after the introduction of different concentrations of carbaryl, and visually observe the addition of different concentrations of carbaryl. After the colo...

Embodiment 2

[0065] A method for quantitatively detecting the pesticide carbaryl in a water matrix based on nano-gold etching, specifically comprising the following steps:

[0066] The carbaryl concentration was (5 × 10 -5 mol / L, 7×10 -5 mol / L, 1×10 -4 mol / L, 1.5×10 -4 mol / L, 2×10 -4 mol / L, 2.5×10 -4 mol / L, 5×10 -4 mol / L) of 50 μL water matrix and 100 μL of the resulting nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution was added to the mixed solution containing 100 μL of the resulting biconical gold nanometer solution (0.9575g / ml) and 150 μL of hydrogen peroxide (3mol / L), and added to the resulting solution system with water The volume is 600μL, react at 40°C for 45min, and then measure the UV-visible spectrum in the range of 400-1000nm, respectively measure (three times in parallel) under the same zinc porphyrin rod concentration, the corresponding nano-gold LSPR peak before and after the introducti...

Embodiment 3

[0071] A method for the quantitative detection of the pesticide carbaryl in a complex matrix based on nano-gold etching. The obtained nano-zinc porphyrin and biconical nano-gold are applied to the detection of carbaryl in fruits, vegetables, Chinese medicinal materials and soil matrices, specifically including Follow the steps below:

[0072] Add 50 μL of carbaryl to a concentration of 7×10 -5 mol / L, 1.5×10 -4 mol / L, 2.5×10 -4 mol / L of different substrates (apple, cabbage, chrysanthemum, lily, Atractylodes macrocephala, soil) and 100 μL of the resulting nano-zinc porphyrin solution (concentration of 9.6×10 - 6mol / L) mixed for 1 min, the mixed solution was added to the mixed solution containing 100 μL of the obtained biconical gold nanometer solution (0.9575 g / ml) and 150 μL of hydrogen peroxide (3mol / L), and supplemented with water to the volume of the obtained solution system was 600 μ L, reacted at 40°C for 45min, then carried out UV-visible spectrometry in the range of ...

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Abstract

The invention discloses a method for detecting pesticide carbaryl based on nanogold etching, which comprises the following steps: adding a to-be-detected solution I containing carbaryl and tetra-(4-pyridyl) zinc porphyrin nanorods into a mixed solution II containing biconical nanogold and hydrogen peroxide, uniformly mixing the solution II and enabling the solution II to react fully to obtain a solution system III; and detecting the carbaryl according to the color change of the solution system III obtained by the reaction before and after introduction of the carbaryl or the peak appearance position change of a corresponding biconical nanogold LSPR peak. The method has the advantages of being easy to operate, high in sensitivity, rich in color change and the like, can achieve rapid, quantitative and visual recognition of carbaryl in food systems with complex components such as fruits, vegetables and traditional Chinese medicinal materials, and is suitable for application and popularization.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a method for detecting the pesticide carbaryl based on nano-gold etching. Background technique [0002] Carbaryl, alias carbaryl, N-methylcarbamate-1-naphthyl ester, (1-naphthyl)-N-methylcarbamate, aminocarbaphthalate bis(2-ethylhexyl) ) ester; has a certain systemic effect, and is often used to control rice planthoppers, leafhoppers, thrips, bean aphids, soybean borers, cotton bollworms, fruit tree pests, forestry pests, etc. In the process of agricultural production, in order to effectively prevent pests and diseases and improve the quality of crop production, during the process of artificially spraying carbaryl, pesticides are absorbed by the roots into the interior of plant tissues and organs, and the pesticides remaining in plant tissues and organs are less affected by the external environment and are difficult to remove by cleaning , and...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/552
CPCG01N21/78G01N21/554
Inventor 付海燕邓高琼韦柳娜王硕陈亨业佘远斌周春松
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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