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Bacteriophage preparation

A bacteriophage and staphylococcus technology, applied in the direction of bacteriophage, virus/bacteriophage, medical raw materials derived from virus/bacteriophage, etc., can solve the problems of high host specificity and no report, and achieve the effect of efficient sterilization, safety and easy use

Pending Publication Date: 2021-06-25
MEIJI SEIKA KAISHA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, no phages with bacteriolytic activity against S. hyicus or S. chromogenes have been reported
In addition, bacteriophages have a problem of high host specificity such that they do not infect bacteria of the same species even if the strains are different.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] (Phages against S. hyicus and S. chromogenes of exudative epidermatitis origin and bacteriolytic activity test)

[0127] As strains for studying bacteriolytic activity, pigs with exudative epidermatitis kept at Meiji Seika Pharma Co., Ltd. Bioindustry Research Institute were used, and many were isolated and selected by conventional methods using mannitol agar medium. A S.hyicus strain and a S.chromogenes strain.

[0128] Bacteria were extracted from the bacterium S.hyicus strain and S.chromogenes strain studied for bacteriolytic activity, suspended in LB medium, and placed in an incubator (37° C.) for 9 hours for bacterial proliferation. In addition, the composition of the LB medium is as follows. That is, in the case of the LB liquid medium, 0.5w / v% of NaCl, 0.5w / w% of yeast extract (Yeast extract), and 1w / v% of polypeptone were contained. In the case of the LB agar medium, 1.5% agar (agar) was further contained in addition to the above-mentioned composition.

...

Embodiment 2

[0138] Furthermore, regarding the phages respectively specified by the deposit number NITE BP-693 and the deposit number NITE BP-694 ( and ), through the same test as above, for Staphylococcus pseudointermediate, Staphylococcus epidermidis, Staphylococcus hemolyticus, Staphylococcus saprophyticus, Staphylococcus sciurinae, Staphylococcus wattleri, Staphylococcus auris, Staphylococcus capitum, Staphylococcus kosheri, Staphylococcus spice, Staphylococcus felis, Staphylococcus hominis, Staphylococcus intermedius, Staphylococcus stutzeri, Staphylococcus mimetic, Staphylococcus xylosus, Staphylococcus alginus were also evaluated for bacteriolytic activity. The obtained results are shown in Tables 16-20.

[0139] Table 16

[0140]

[0141] Table 17

[0142]

[0143] Table 18

[0144]

[0145] Table 19

[0146]

[0147] Table 20

[0148]

[0149] As can be seen from the results shown in Tables 16 to 20, and The above-mentioned Staphylococcus also exhibited ...

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Abstract

It is found that each of a bacteriophage identified by Accession No. NITE BP-693 and a bacteriophage identified by Accession No. NITE BP-694 has a bacteriolytic activity against Staphylococcus hyicus, Staphylococcus chromogenes and the like. This finding enables the provision of: a composition for lysing these staphylococcal bacteria; a composition for treating or preventing diseases associated with these staphylococcal bacteria; and others.

Description

technical field [0001] The present invention relates to a composition for lysing staphylococci, comprising, as an active ingredient, a bacteriophage having a property of lysing staphylococci (S. suis, staphylococcus chromogenes, etc.). Furthermore, the present invention relates to the prophylactic or therapeutic use of the composition for diseases caused by the staphylococcus. In addition, the present invention relates to a composition for detecting said staphylococcus comprising said phage. Background technique [0002] Staphylococcus hyicus (S. hyicus), Staphylococcus chromogenes (S. chromogenes), etc. which are a type of staphylococci cause various diseases to humans and animals. Exudative epidermatitis is known as one of skin diseases caused by bacteria such as S. hyicus and S. chromogenes (Non-Patent Documents 1 to 3, Patent Document 1). Exudative epidermatitis is called "oily skin disease". The whole body of piglets is covered with oily skin. The disease usually occu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/76A61P17/00A61P31/04
CPCA61P17/00A61P31/04C12N7/00C12N2795/10121C12N2795/10131C12N2795/10132A61K35/76
Inventor 岩野英知樋口豪纪田村丰臼井优藤木纯平
Owner MEIJI SEIKA KAISHA LTD