Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of carbamazepine antigen and preparation method thereof

A carbamazepine and antigen technology, which is applied in the field of carbamazepine antigen and its preparation, can solve the problems of being unsuitable for batch sample screening detection and on-site instant detection, expensive and other problems, and achieves high product yield, strong specificity, and preparation process. simple steps

Active Publication Date: 2022-05-10
HANGZHOU CLONGENE BIOTECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods have extremely high sensitivity and precision, but require expensive instruments, equipment and specially trained technicians, and are not suitable for screening and on-site detection of batch samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of carbamazepine antigen and preparation method thereof
  • A kind of carbamazepine antigen and preparation method thereof
  • A kind of carbamazepine antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] A preparation method for carbamazepine antigen, comprising the following steps:

[0033] (1) Synthesis of 10-methoxy-5H-dibenzo[b, f]azepine-5-carboxamide (II)

[0034]

[0035] Under nitrogen protection, dissolve 10-methoxy-5H-dibenzo(b,f)azepine (I) in dichloromethane, lower the temperature to 0-5°C, add chlorosulfonyl isocyanate, the The mass ratio of carbamazepine and chlorosulfonyl isocyanate is 1:0.8-1. After the addition, the temperature is raised to 20-30° C. for 12-18 hours. Add water, adjust the pH to 8-9 with 1N sodium hydroxide solution, add dichloromethane, extract and separate layers, wash the organic layer with water, dry the organic layer over anhydrous sodium sulfate, and concentrate under reduced pressure to obtain 10-methoxy-5H-diphenyl And [b, f] azepine-5-carboxamide (II).

[0036] (2) Synthesis of 10-hydroxyl-5H-dibenzo[b, f]azepine-5-carboxamide (III)

[0037]

[0038] Under nitrogen protection, dissolve 10-methoxy-5H-dibenzo[b,f]azepine-...

Embodiment 1

[0046] A preparation method for carbamazepine antigen, comprising the following steps:

[0047](1) Synthesis of 10-methoxy-5H-dibenzo[b, f]azepine-5-carboxamide (II)

[0048] Under nitrogen protection, dissolve 45mg of 10-methoxy-5H-dibenzo(b,f)azepine (I) in 10mL of dichloromethane, lower the temperature to 0-5°C, add 40mg of chlorosulfonyl isocyanate, After the addition, the temperature was raised to 25°C for 15 hours. Add 10 mL of water, adjust the pH to 8.2 with 1N sodium hydroxide solution, add 30 mL of dichloromethane, extract and separate layers, wash the organic layer with 10 mL of water, dry the organic layer over anhydrous sodium sulfate, and concentrate under reduced pressure to obtain 58 mg of 10-methoxy- 5H-Dibenzo[b,f]azepine-5-carboxamide (II).

[0049] (2) Synthesis of 10-hydroxyl-5H-dibenzo[b, f]azepine-5-carboxamide (III)

[0050] Under nitrogen protection, 58mg of 10-methoxy-5H-dibenzo[b,f]azepine-5-carboxamide (II) was dissolved in 30mL of dichloromethan...

Embodiment 2

[0056] A preparation method for carbamazepine antigen, comprising the following steps:

[0057] (1) Synthesis of 10-methoxy-5H-dibenzo[b, f]azepine-5-carboxamide (II)

[0058] Under nitrogen protection, dissolve 45mg of 10-methoxy-5H-dibenzo(b, f)azepine (I) in 12mL of dichloromethane, lower the temperature to 0-5°C, add 41mg of chlorosulfonyl isocyanate After the addition, the temperature was raised to 25°C for 16h. Add 10 mL of water, adjust the pH to 8.7 with 1N sodium hydroxide solution, add 40 mL of dichloromethane, extract and separate layers, wash the organic layer with 10 mL of water, dry the organic layer over anhydrous sodium sulfate, and concentrate under reduced pressure to obtain 59 mg of 10-methoxy- 5H-Dibenzo[b,f]azepine-5-carboxamide (II).

[0059] (2) Synthesis of 10-hydroxyl-5H-dibenzo[b, f]azepine-5-carboxamide (III)

[0060] Under nitrogen protection, dissolve 59mg of 10-methoxy-5H-dibenzo[b,f]azepine-5-carboxamide (II) in 30mL of dichloromethane, lower ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the field of antigens. The present invention discloses a carbamazepine antigen and a preparation method thereof. The preparation method of the carbamazepine antigen comprises: (1) treating 10-methoxy-5H-diphenyl with chlorosulfonyl isocyanate And (b, f) azepine is subjected to amidation reaction to obtain 10-methoxy-5H-dibenzo[b, f] azepine-5-carboxamide; (2) 10-methoxy- 5H‑Dibenzo[b, f]azepine‑5‑carboxamide Cleave the ether bond with boron tribromide to give 10‑hydroxy‑5H‑dibenzo[b, f]azepine‑5‑ formamide; (3) react 10‑hydroxy‑5H‑dibenzo[b, f]azepine‑5‑carboxamide with succinic anhydride to obtain a carboxyl-containing hapten; (4) react carbamazepine After the antigen is coupled with the carrier protein, the carbamazepine antigen is obtained. The carbamazepine antigen of the invention relatively completely retains the molecular structure of carbamazepine, so it has higher specificity and sensitivity.

Description

technical field [0001] The invention relates to the field of antigens, in particular to a carbamazepine antigen and a preparation method thereof. Background technique [0002] Carbamazepine, scientific name "5H-dibenzo[b,f]azepine-5-carboxamide", its structural formula is as follows: [0003] [0004] Carbamazepine is a dibenzoazepine antiepileptic drug. It is mainly used in the treatment of epilepsy, central nervous uremia, antimanic depression, and is also commonly used in antiarrhythmia. Because carbamazepine is relatively safe and effective, it has become the first choice of antiepileptic drugs for most people. [0005] Because carbamazepine is widely used, its adverse reactions have been paid more and more attention. Some studies have found that taking carbamazepine in large quantities for a long time can cause damage to liver and kidney function, leading to renal failure; patients who take carbamazepine often have allergic reactions such as headache, headache, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K14/77C07K14/795C07K14/47C07K1/34
CPCC07K14/765C07K14/77C07K14/795C07K14/4717
Inventor 曾繁荣郑曙剑
Owner HANGZHOU CLONGENE BIOTECH