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Fusion protein nano vaccine based on RAS variant neoantigen epitopes and preparation method thereof

A fusion protein and antigen epitope technology, applied in the direction of fusion polypeptide, cancer antigen components, chemical instruments and methods, etc., can solve the problems of antigen dispersion, unfavorable immune system recognition and activation, etc.

Pending Publication Date: 2021-07-06
江苏欣生元生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptide vaccines are highly safe, but the antigens are too scattered, which is not conducive to the recognition and activation of the immune system
However, mRNA vaccines have other problems such as delivery and storage, and the efficiency of transcribing into polypeptide antigens in vivo

Method used

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  • Fusion protein nano vaccine based on RAS variant neoantigen epitopes and preparation method thereof
  • Fusion protein nano vaccine based on RAS variant neoantigen epitopes and preparation method thereof
  • Fusion protein nano vaccine based on RAS variant neoantigen epitopes and preparation method thereof

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Embodiment Construction

[0020] 1) Mutated epitope sequence

[0021] In recent years, with the popularization of gene sequencing, many gene variations that affect the occurrence and development of pancreatic cancer have been identified. The 21kD guanine nucleotide-binding protein RAS (p21RAS) encoded by the human proto-oncogene family is an important member of the cell growth signaling chain. In tumor cells, a single amino acid substitution occurs at position 12 or 13 due to a single nucleic acid mutation in the RAS gene. This mutation leaves RAS in a state of constant signaling activation, which leads to uncontrolled cell growth and cancerous transformation.

[0022] Studies have shown that multiple RAS mutations may be present in the same neoplastic pancreas. Although in pancreatic cancer, RAS mutations mainly occur at position 12, there are also some degree of mutations at positions 13 and 61. These variant antigens represent more than 99% of mutations in RAS proteins in common pancreatic cancer...

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Abstract

The invention discloses a fusion protein nano vaccine based on RAS variant neoantigen epitopes, and belongs to the field of therapeutic tumor vaccines. The 'antigen epitope fusion protein' vaccine comprises at least four RAS neoantigens that can cause human cell carcinogenesis, and an adjuvant capable of stimulating antibody production and T cell response is added. The vaccine can be applied through subcutaneous or intramuscular injection, and cancerous cells with variant RAS can be accurately recognized and killed by the generated killing T cells against the variant RAS neoantigens, so that the aim of treating tumors is fulfilled.

Description

technical field [0001] The invention belongs to the field of immunotherapeutic vaccines, and relates to a fusion protein vaccine formed by a combination of multiple tumor neonatal antigen epitopes produced by specific RAS gene mutation. Background technique [0002] Tumor neoantigen is a kind of polypeptide fragment with host antigenicity in the variant protein sequence caused by tumor cell gene mutation. Such neoantigens can be recognized by the host immune system as "foreign" antigens and activate specific immune responses. Therapeutic vaccines based on tumor "neoantigens" can recognize and kill tumors by activating the immune cells of neoantigens that may exist in the patient's body. At the same time, anti-tumor immune memory can also be established through active immunization. [0003] Most tumor neoantigens have strong individual characteristics. From the perspective of gene variation, different tumors will have different protein target mutations, and the same protei...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39A61K9/51A61K47/36C07K19/00C12N15/70A61P35/00
CPCC07K14/82C12N15/70A61K39/0011A61K39/39A61P35/00A61K9/5161C07K2319/00A61K2039/55561
Inventor 王建新顾云张聿恒
Owner 江苏欣生元生物科技有限公司