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Method for constructing diabetic zebrafish model

A technology for zebrafish and diabetes, applied in the field of biological modeling, can solve the problems of building a diabetic zebrafish model, long modeling time, long culture period, etc., achieving excellent application prospects, low cost of consumables, modeling short time effect

Active Publication Date: 2021-07-09
WENZHOU UNIVERSITY
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Problems solved by technology

[0003] However, the main disadvantages of the current zebrafish model are high mortality and long modeling time. These defects are also the main problems that have not been overcome in modeling.
Patent CN201810767264.8 discloses a breeding method and device for improving the survival rate of zebrafish juveniles. The breeding method can significantly improve the survival rate of zebrafish juveniles with a specific device, but the culture period is longer. And did not give the inspiration to construct the diabetic zebrafish model
[0004] Therefore, there is an urgent need for a diabetic zebrafish model to be used in the research of diabetes-related drugs, and to overcome the above-mentioned technical problems such as high mortality and long modeling time.

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  • Method for constructing diabetic zebrafish model
  • Method for constructing diabetic zebrafish model
  • Method for constructing diabetic zebrafish model

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specific Embodiment

[0031] 1. Experimental animals

[0032] Zebrafish: wild type, Institute of Hydrobiology, Chinese Academy of Sciences (adult healthy zebrafish over 5 months old; 14 hours of light per day, 10 hours of darkness); the requirements for healthy zebrafish here: blood sugar index is normal (that is, blood sugar The concentration is between 2.8mmol / L-6.1mmol / L).

[0033] Hatchable eggs of Artemia (stored at -20°C): Tianjin Fengnian Aquaculture Co., Ltd.

[0034] 2. Experimental reagents

[0035] Glucose (analytical pure): Tianjin Beilian Fine Chemicals Development Co., Ltd.;

[0036] Artemia hatching salt: Tianjin Fengnian Aquaculture Co., Ltd.;

[0037] Cool tap water after boiling.

[0038] 3. Experimental equipment

[0039] Artemia incubator: the volume is 1700ml, (Jian Keshan Technology Co., Ltd.);

[0040] Circulating water system for zebrafish breeding: water temperature 28°C, pH 7-8, conductivity: 500-800μS / cm, salinity: 0.25-0.5% (Shanghai Feixi Biotechnology Co., Ltd.);...

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Abstract

The invention relates to the field of biological modeling, in particular to a method for constructing a diabetic zebrafish model. According to the method, the content of sugar in zebrafish feed is increased to induce the construction of the diabetic zebrafish model, so that the certainty of the administration dosage is realized. The death rate of modeling is low, the modeling time is short, the administration dosage can be controlled, the method is simple and convenient, the cost of consumables needed in the modeling process is low, and the method has good application prospects.

Description

technical field [0001] The invention relates to the field of biological modeling, in particular to a method for constructing a diabetic zebrafish model. Background technique [0002] Diabetes includes many types. Clinically, the fasting blood glucose content exceeds 7.1mmol / L as the index. In the prior art, the zebrafish diabetes model is often used to study the efficacy of hypoglycemic drugs. Lao Qiaocong et al. Zebrafish Diabetes Model Evaluation of Commonly Used Hypoglycemic Drugs Hypoglycemic Efficacy" Select wild-type AB line zebrafish larvae, use alloxan combined with glucose as inducer to establish zebrafish diabetes model; use this model to evaluate metformin, acarbose Hypoglycemic efficacy of sugar, sitagliptin and glibenuria. [0003] However, the main disadvantages of the current zebrafish model are high mortality and long modeling time, and these defects are also the main problems that have not been overcome in modeling. Patent CN201810767264.8 discloses a bree...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/59A01K61/17
CPCA01K61/10A01K61/59A01K61/17Y02A40/81
Inventor 王磊孙达金立波彭仁义李瑶琦
Owner WENZHOU UNIVERSITY
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