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Detection chip for detecting male malignant tumor marker, and production method and application thereof

A malignant tumor and detection chip technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as functional differences, and achieve the effects of preventing false positives, eliminating non-specific adsorption, and low background value

Pending Publication Date: 2021-07-16
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two subtypes of SCCA, namely SCCA1 and SCCA2. The gene nucleic acid sequence of the two is 98% homologous, and the protein amino acid sequence is 92% identical, but there are large differences in function

Method used

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  • Detection chip for detecting male malignant tumor marker, and production method and application thereof
  • Detection chip for detecting male malignant tumor marker, and production method and application thereof
  • Detection chip for detecting male malignant tumor marker, and production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Experiment setup method

[0035] The tested antibodies used in this example are shown in Table 1, and the suppliers involved are as follows: Guangzhou Ruiboao Biotechnology Co., Ltd. (Ruiboao), Santa Cruz Biotechnology (Santa Cruz), Thermo Fisher Technology (invitrogen), Novus Biologicals (Novusbio), Faipeng Biological Co., Ltd. (Fepeng), RDBiosciences (RD), Shanghai Yiji Industrial Co., Ltd. Le), Nanjing Oukai Biotechnology Co., Ltd. (Oukai), OriGene Technologies (Origene), GenScript Biotechnology Co., Ltd. (GenScript), Parker Bio (Parker). At 37°C, using Thermo Scientific TM Wellwash TM The plate washer was used to make the tested antibodies react with AFP, CA125, TG, CA199, CA724, CEA, fPSA, tPSA, NSE, PGI, PGII, and SCCA1 to carry out the matching test of the tested antibodies and antigens.

[0036] The experimental parameters of the paired detection experiment are: carrier, 96 microwell plate; antigen diluent, PBS; blocking solution, 5% skimmed milk powder +...

Embodiment 2

[0086] 1. Test antibody

[0087] Select the following antibodies in Example 1 as the test antibody of this example:

[0088] The first AFP antibody, the third AFP antibody, the sixth AFP antibody;

[0089] the second CA125 antibody, the third CA125 antibody, the sixth CA125 antibody;

[0090] The second TG antibody, the fourth TG antibody, the fifth TG antibody;

[0091] The first CEA antibody, the fourth CEA antibody;

[0092] Second fPSA antibody, fourth fPSA antibody, fifth fPSA antibody, seventh fPSA antibody;

[0093] Second tPSA antibody, fifth tPSA antibody;

[0094] The second NSE antibody, the fourth NSE antibody, the fifth NSE antibody;

[0095] The first PGI antibody, the third PGI antibody;

[0096] The first PGII antibody, the second PGII antibody, the third PGII antibody, the fifth PGII antibody;

[0097] First SCCA1 antibody, second SCCA1 antibody, fourth SCCA1 antibody, sixth SCCA1 antibody.

[0098] 2. Experiment setting method

[0099] The test antib...

Embodiment 3

[0126] 1. Experiment setup method

[0127] In this example, the solid-phase carrier of the chip for preparing the capture antibody mixture is used as a variable to set up two treatment groups. The chip is used as the solid phase carrier of the chip.

[0128] The primary antibody combinations used in this example are those in Example 1: the third AFP antibody, the sixth CA125 antibody, the fifth TG antibody, the first CEA antibody, the first CA199 antibody, the first CA724 antibody, the seventh fPSA antibody, Fifth tPSA antibody, second tPSA antibody, second NSE antibody, third PGI antibody, first PGII antibody, and second SCCA1 antibody.

[0129] The secondary antibody combinations used in this example are those in Example 1: the first AFP antibody, the second CA125 antibody, the fourth TG antibody, the fourth CEA antibody, the second CA199 antibody, the second CA724 antibody, the fifth fPSA antibody, Second tPSA antibody, fifth tPSA antibody, fourth NSE antibody, first PGI ...

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Abstract

The invention provides a detection chip for detecting a male malignant tumor marker, and a production method and an application thereof. The detection chip for detecting the male malignant tumor marker comprises a solid phase carrier and a capture antibody fixed on the surface of the solid phase carrier; the capture antibody comprises at least two of an anti-AFP-capture antibody, an anti-CA125-capture antibody, an anti-TG-capture antibody, an anti-CA199-capture antibody, an anti-CA724-capture antibody, an anti-CEA-capture antibody, an anti-fPSA-capture antibody, an anti-tPSA-capture antibody, an anti-NSE-capture antibody, an anti-PGI-capture antibody, an anti-PGII-capture antibody and an anti-SCCA1-capture antibody; and the solid-phase carrier is selected from an aminated slide or an aldehyde slide. According to the invention, the specific antibodies aiming at the 12 tumor markers at most can be stably fixed on the surface of the same solid-phase carrier so that high-throughput detection of the 12 male tumor markers at most can be realized at the same time.

Description

technical field [0001] The invention belongs to the technical field of in vitro immune analysis, and in particular relates to a detection chip for detecting male malignant tumor markers, a preparation method and application thereof. Background technique [0002] Cancer, also known as malignant neoplasm in medical terms, is a disease caused by abnormalities in the mechanism of controlling cell growth and proliferation. In addition to growing uncontrollably, cancer cells can also partially invade surrounding normal tissues (invasion) and even metastasize to other parts of the body through the internal circulatory system or lymphatic system. Cancer is a multifactorial disease. Its etiology may be related to heredity, gene mutation, aging, or environmental factors. Due to the high morbidity and mortality, it seriously threatens the health of the public. [0003] In 2018, there were 9,456,418 new cancer cases in men worldwide, with an incidence rate of 245.6 / 100,000 and ASIRW ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/552
CPCG01N33/57476G01N33/5748G01N33/57492G01N33/57473G01N33/57484G01N33/552
Inventor 吕志强胡守旺黄若磐
Owner RAYBIOTECH INC GUANGZHOU