Application of tripterygium wilfordii extract component liposome in preparation of drug for preventing and treating lipopolysaccharide-induced behavioral cognitive disorder through nasal delivery
A technique of extracting ingredients and nasal administration, which is applied in the field of medicine to achieve the effect of alleviating behavioral cognitive impairment, huge social and economic benefits, and inhibiting the inflammatory response of brain nerves
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0016] In the specific implementation of the present invention, described Tripterygium wilfordii extract component liposome is:
[0017] (1) Weigh 40 parts by weight of soybean lecithin, 10 parts of cholesterol and 1 part of tripterygium glycosides, ultrasonically dissolve it in dichloromethane, an organic solvent, for 5-10 minutes, and evaporate in a constant temperature water bath at 40°C at 20 rpm for 20 minutes , to obtain a uniform lipid film;
[0018] (2), dry the lipid film under vacuum for 4 hours, add 25,000 parts of phosphate buffer saline PBS with a weight-to-volume ratio, stir at room temperature for 4 hours, place in 100w ultrasonic for 5 minutes, and then pass through 200nm and 100nm filters successively with a manual squeezer Membrane, after secondary ultrafiltration with a 50000MWCO ultrafiltration tube, take the supernatant, which is tripterygium glycosides liposomes;
[0019] The weight-to-volume ratio is calculated in grams for solids and liters for liquids...
Embodiment 2
[0021] In the specific implementation of the present invention, the tripterygium wilfordii extract component liposome is:
[0022] (1) Weigh 40 parts by weight of soybean lecithin, 10 parts of cholesterol and 1 part of triptolide, ultrasonically dissolve it in dichloromethane, an organic solvent, for 5-10 minutes, and evaporate under reduced pressure at 20 rpm for 20 minutes in a constant temperature water bath at 40°C , to obtain a uniform lipid film;
[0023] (2), dry the lipid film under vacuum for 4 hours, then add 25,000 parts of phosphate buffered saline PBS with a weight-to-volume ratio (same as above), stir at room temperature for 4 hours, place 100w ultrasound for 5 minutes, and then use a manual extruder to pass through 200nm, 100nm filter membrane, after secondary ultrafiltration with 50000MWCO ultrafiltration tube, take the supernatant, which is triptolide liposome.
Embodiment 3
[0025] In the specific implementation of the present invention, the tripterygium wilfordii extract component liposome is:
[0026] (1) Weigh 40 parts by weight of soybean lecithin, 10 parts of cholesterol and 1 part of tripterine, ultrasonically dissolve it in dichloromethane, an organic solvent, for 5-10 minutes, and evaporate under reduced pressure at 20 rpm in a constant temperature water bath at 40°C for 20 minutes , to obtain a uniform lipid film;
[0027] (2), dry the lipid film under vacuum for 4 hours, then add 25,000 parts of phosphate buffered saline PBS with a weight-to-volume ratio (same as above), stir at room temperature for 4 hours, place 100w ultrasound for 5 minutes, and then use a manual extruder to pass through 200nm, 100nm filter membrane, after secondary ultrafiltration with 50000MWCO ultrafiltration tube, take the supernatant, which is tripterine liposome.
[0028] According to the above-mentioned method, any amount of tripterygium extract component lipo...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More