Colloidal gold test strip for detecting human thyroglobulin

A technology of thyroglobulin and test strips, applied in the field of biomedical testing, can solve the problems of large impact, long time, complicated operation, etc., and achieve the effects of convenient and fast use, reduced workload, and shortened testing time

Active Publication Date: 2021-07-23
施康培医药科技(武汉)有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

This detection method is complicated to operate and takes a long time, which is not conducive to clinical operation.
Third, there may be auto-Tg antibodies in peripheral blood, and the presence of auto-Tg antibodies has a greater impact on the quantitative detection of Tg

Method used

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  • Colloidal gold test strip for detecting human thyroglobulin
  • Colloidal gold test strip for detecting human thyroglobulin
  • Colloidal gold test strip for detecting human thyroglobulin

Examples

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Embodiment Construction

[0026] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0027] 1. Antibody screening and preparation

[0028] Full-length thyroglobulin (Tg) was used to immunize alpacas to prepare anti-human Tg antibodies. Mix and emulsify 250 μg antigen with 250 μL Freund’s complete adjuvant, and use the mixture to immunize alpacas to complete the primary immunization. After 2 weeks and 4 weeks after the primary immunization, the alpacas were given a booster immunization, and the mixture of 1250 μg antigen and 250 μL Freund's incomplete adjuvant was used for booster immunization. One week after the second and third immunizations, blood was collected to determine the titer of antiserum.

[0029] The results showed that the antiserum titers elicited by the second and third immunizations of the antigen were 2.25×10 6 and 9.43×10 ...

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Abstract

The invention relates to a test strip for detecting human thyroglobulin, which comprises a bottom plate, and a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and connected in sequence; a detection line is arranged on the nitrocellulose membrane; the colloidal gold combination pad contains colloidal gold particles labeled by a combined antibody; a capture antibody is anchored in the detection line, and the binding antibody and the capture antibody can specifically recognize and bind thyroglobulin and do not interfere with each other. The colloidal gold test strip provided by the invention is convenient and rapid to use, can complete a chromatographic reaction within 10 minutes, is used for quantitative detection, and helps a doctor to judge whether the Tg content in peripheral blood exceeds a normal value range or not within a short time. The semi-quantitative detection test strip can be used by a patient, and daily self-monitoring of the patient is facilitated. The bifunctional test strip can be used for removing test samples with Tg content in a normal range, so that the workload of clinical detection is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a colloidal gold test strip for detecting human thyroglobulin. Background technique [0002] Thyroglobulin (Tg) is a glycoprotein secreted by thyroid follicular epithelial cells, with a molecular weight of about 660kD. play an important role in the biosynthesis of . In a healthy person, only a very small amount of Tg enters the blood circulation, and the recommended normal value of Tg is 3-40ng / ml. Some diseases such as differentiated thyroid cancer (DTC) can cause elevated Tg levels in peripheral blood. Therefore, Tg is a key serological indicator of DTC, which can assist in the initial diagnosis and diagnosis of DTC. Regular follow-up of Tg levels after treatment can assist in monitoring the prognosis and recurrence risk of DTC patients. [0003] At present, there are some technical problems in the determination of Tg content. First of all, there a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/558G01N33/58G01N33/68G01N33/577
CPCG01N33/558G01N33/57407G01N33/57488G01N33/577G01N33/587G01N33/68G01N2333/4713
Inventor 朱富强王珏
Owner 施康培医药科技(武汉)有限公司
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