Method for improving germination rate of traditional Chinese medicinal material astragalus membranaceus seeds

A technology of astragalus seeds and germination rate, applied in the directions of seed immunity, seed and rhizome treatment, application, etc., can solve problems such as easy occurrence of danger, and achieve the effects of speeding up the breeding process, shortening the breeding cycle, and high survival rate.

Pending Publication Date: 2021-07-30
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for improving the germination rate of the Chinese medicinal material Astragalus membranaceus seeds, solve the problem that the use of sulfuric acid to soak the seeds is prone to danger, and simultaneously improve the problem of the germination rate of the Astragalus membranaceus seeds

Method used

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  • Method for improving germination rate of traditional Chinese medicinal material astragalus membranaceus seeds
  • Method for improving germination rate of traditional Chinese medicinal material astragalus membranaceus seeds
  • Method for improving germination rate of traditional Chinese medicinal material astragalus membranaceus seeds

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Effect test

Embodiment 1

[0031] Embodiment 1: Astragalus seed germination method

[0032] (1) Seed selection: select astragalus seeds with full grain and uniform size;

[0033] (2) Disinfection treatment: the seeds are rinsed repeatedly with distilled water for 2-3 times, then soaked in 2wt% sodium hypochlorite solution for 15 minutes, and then cleaned with distilled water for 4-5 times;

[0034] (3) Bacterial solution infection: arrange the sterilized seeds evenly and neatly in a petri dish for dipping, the dosage is subject to soaking but not submerging the seeds, and the test is repeated 3 times;

[0035] (4) Drying treatment: place the dyed seeds in an oven at 30°C for 4.5 hours until the seeds are completely dry;

[0036] (5) Cultivation and germination: the seeds were placed in a petri dish lined with double-layer filter paper, and the petri dish was placed under the conditions of 25° C. and a relative humidity of 80% for 12 hours of light and 12 hours of darkness for constant temperature and h...

Embodiment 2

[0050] Embodiment 2: Astragalus seed germination method

[0051] (1) Seed selection: select the Astragalus mongolica seeds with plump grains and uniform size for pretreatment, wash the Astragalus seeds with 2wt% sodium hydroxide solution, rinse them with water, and then mix 15wt% urea solution with river sand according to the Mix at a mass ratio of 1:0.2, add astragalus seeds and stir at a stirring rate of 300rpm for 8 minutes, take it out, clean it and dry it;

[0052](2) Disinfection treatment: the seeds were rinsed repeatedly with distilled water for 2-3 times, then soaked in 2wt% sodium hypochlorite solution for 15 minutes, and then washed with distilled water for 4-5 times, followed by gel electric stimulation treatment. Mix and heat agarose, cornstarch and distilled water at a weight ratio of 1:0.5:100, boil until transparent and cool to 55°C, add sterilized Astragalus seeds, the total mass ratio of Astragalus seeds to gel is 0.5:1, Then they were placed under hyperoxia...

Embodiment 3

[0056] Embodiment 3: Astragalus seed germination method

[0057] (1) Seed selection: select full grains, and the Astragalus membranaceus seeds of uniform size are pretreated, and the Astragalus seeds are washed with 2wt% sodium hydroxide solution, rinsed with water, and then 15wt% urea solution and river sand Mix according to the mass ratio of 1:0.2, add astragalus seeds and stir at a stirring rate of 200rpm for 6 minutes, take it out, clean it and dry it;

[0058] (2) Disinfection treatment: the seeds were rinsed repeatedly with distilled water for 2-3 times, then soaked in 2wt% sodium hypochlorite solution for 15 minutes, and then washed with distilled water for 4-5 times, followed by gel electric stimulation treatment. Mix and heat agarose, cornstarch and distilled water at a weight ratio of 1:0.5:100, boil until transparent, then cool to 45°C, add sterilized Astragalus seeds, the total mass ratio of Astragalus seeds to gel is 0.5:1, Then they were placed under hyperoxia c...

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Abstract

The invention discloses a method for improving the germination rate of traditional Chinese medicinal material astragalus membranaceus seeds, and belongs to the technical field of astragalus membranaceus seed germination. The method comprises the following steps that (1), seeds are selected, specifically, astragalus membranaceus seeds with full grains and uniform size are selected; (2), disinfection treatment is carried out, specifically, the seeds are repeatedly washed with distilled water for 2-3 times, then soaked with a 2wt% sodium hypochlorite solution for 15 minutes, and then washed with distilled water for 4-5 times; (3), bacterial liquid infection is carried out, specifically, sterilization treatment is carried out on the EM bacterial liquid, and then the disinfected seeds are dip-dyed; (4), drying treatment is carried out, specifically, the dip-dyed seeds are dried in a drying oven at 30 DEG C for 4.5 hours until the seeds are completely dried; and (5), cultivation and germination are carried out, specifically, the seeds are placed in a culture dish padded with double-layer filter paper, constant-temperature culture is carried out on the culture dish, the problem that danger is prone to happening when sulfuric acid is used for soaking the seeds is solved, and meanwhile, the germination rate of the astragalus membranaceus seeds is improved.

Description

technical field [0001] The invention relates to the technical field of astragalus seed germination, in particular to a method for improving the germination rate of astragalus seeds, a traditional Chinese medicinal material. Background technique [0002] Astragalus (scientific name: Astragalus propinquus Schischkin) is a plant of the genus Astragalus in the leguminous family. Perennial herb, 50-100 cm tall. The main root is thick, woody, often branched, off-white. Among them, Astragalus membranaceus and Astragalus membranaceus are the main cultivated species in my country, and Astragalus membranaceus is a variant of Astragalus membranaceus. Astragalus membranaceus and Astragalus mongolica often have difficulty in seed germination during planting. The seeds of Astragalus membranaceus are small, and the hilum is also small, which hinders the water absorption of the seeds, and the seed coat of Astragalus seeds has a dense cuticle and closely arranged palisade cells, and the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 胡国富杨劼宫婷卢宝伟徐松滨秦立刚张攀梅琳琳林宇龙刘洪强王琪李韦遥刘微
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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