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Application of citric acid in preparation of medicine for preventing and/or treating autoimmune diseases

An autoimmune disease, citric acid technology, applied in the application field of medicine, can solve the problems of abnormal liver and kidney function, accompanying infection, etc., and achieve the effect of inhibiting cell differentiation, reducing the incidence rate, and reducing the score of arthritis

Active Publication Date: 2021-07-30
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of hormones, immunosuppressants, and biological agents to suppress the immune response is usually accompanied by adverse reactions such as infection, abnormal liver and kidney function, and anemia

Method used

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  • Application of citric acid in preparation of medicine for preventing and/or treating autoimmune diseases
  • Application of citric acid in preparation of medicine for preventing and/or treating autoimmune diseases
  • Application of citric acid in preparation of medicine for preventing and/or treating autoimmune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Serum and urine citric acid levels are significantly reduced in patients with rheumatoid arthritis

Embodiment approach

[0031] (1) Collection of urine samples and serological samples

[0032] Serum and urine were collected from healthy controls (HC, n=36) and patients with rheumatoid arthritis (RA, n=42). In this study, 42 patients with rheumatoid arthritis who were treated at Peking University People's Hospital from March 2018 to May 2018 were consecutively included. All patients met the 1987 American College of Rheumatology classification criteria for rheumatoid arthritis or early rheumatoid arthritis. (ERA) criteria or the 2010 American College of Rheumatology / European League Against Rheumatism classification criteria for rheumatoid arthritis, and 36 sex- and age-matched healthy volunteers were included.

[0033]1) Instruct the subjects to take clean midstream urine, keep 45mL, divide into three 15mL sterile centrifuge tubes, and send them to the laboratory for storage in a -80°C refrigerator within 1 hour.

[0034] 2) On the day when the urine sample was collected, blood was collected veno...

Embodiment 2

[0044] citric acid inhibition T cells differentiate into pro-inflammatory cells Th17 and promote their differentiation into anti-inflammatory T cells Treg

[0045] method of execution

[0046] (1) Sorting T cells: separate the lymph node or spleen of the mouse, and place the lymph node in 1640 medium containing 10% fetal bovine serum, cut it into pieces with scissors, and grind it with a filter to make a single cell suspension. Spleen: Place the spleen in 1640 medium containing 10% fetal bovine serum, and grind it with a filter to make a single cell suspension. Add 6 mL of erythrocyte lysate, mix evenly by inversion, and place at room temperature for 10 min. Normal temperature, 1800rpm, centrifuge for 8min, discard the supernatant. Lymph node and spleen cells were washed twice with PBS buffer, filtered with a blue cap flow tube, and placed on ice for later use.

[0047] (2) Sorting by flow cytometry T cells (CD4+CD62L+CD44-).

[0048] (3) Coat anti-CD3 and anti-CD28 ...

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PUM

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Abstract

The invention provides application of citric acid in preparation of a medicine for preventing and / or treating autoimmune diseases, and belongs to the technical field of autoimmune disease treatment. The citric acid has the effects of promoting the differentiation of an anti-inflammatory cell-Treg cell and inhibiting the differentiation of a pro-inflammatory cell-Th17 cell; citric acid is used for intervening collagen-induced arthritis model mice, so that the arthritis score of the mice can be remarkably reduced, and the morbidity of the arthritis mice can be reduced; the citric acid can correct the immune cell imbalance state in a living body, and provides a new treatment scheme for rheumatoid arthritis and other autoimmune diseases.

Description

technical field [0001] The invention belongs to the technical field of autoimmune diseases, and in particular relates to the application of citric acid in the preparation of medicines for preventing and / or treating autoimmune diseases. Background technique [0002] Autoimmune diseases are a group of diseases characterized by the appearance of multiple pathogenic autoantibodies in the serum and the involvement of multiple systems throughout the body, including rheumatoid arthritis (RA), systemic lupus erythematosus (systemic lupus erythematosus, SLE), Systemic Sclerosis (SSc), etc. Rheumatoid arthritis is a chronic inflammatory autoimmune disease characterized by autoantibody production and joint destruction. Involvement of external organs, including the heart, lungs, kidneys, nervous system and other systemic organs. According to statistics, RA is one of the diseases with the highest disability rate, which seriously affects human health and brings huge economic burden to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/194A61P37/02A61P19/02A61P29/00A61P25/00A23K20/10A23K50/50
CPCA61K31/194A61P37/02A61P19/02A61P29/00A61P25/00A23K20/10A23K50/50
Inventor 栗占国靳家扬李静贾汝琳
Owner PEOPLES HOSPITAL PEKING UNIV
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