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In-vitro regeneration method for caragana korshinskii

A caragana caragana, in vitro regeneration technology, applied in the field of plant tissue culture and biology, can solve the problems of lack of efficient in vitro regeneration system, limited genetic transformation and the like

Active Publication Date: 2021-08-03
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, the in vitro regeneration of Caragana japonica is still in the trial stage, and the key factor limiting its genetic transformation is the lack of an efficient in vitro regeneration system.

Method used

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  • In-vitro regeneration method for caragana korshinskii
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  • In-vitro regeneration method for caragana korshinskii

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Experimental program
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Embodiment 1

[0029] (1) Preparation of explant material

[0030] Select Caragana seeds with full grains, no insect eyes, and no black spots, wash them with detergent for 3 minutes, wrap them with 75% recycled alcohol cotton to wipe the seed coat, and use recycled alcohol for operation in the ultra-clean bench, and then clean them with clean water. Disinfect with 75% alcohol for 2 minutes, and finally disinfect with 10% sodium hypochlorite for 20 minutes, wash with sterile water for 5 to 6 times, blot the surface moisture with sterile filter paper, inoculate into MS basic medium, and cultivate for about 10 days to obtain newly germinated seeds. Among them, 30g / L sucrose, 0.1g / L inositol and 5.5g / L agar powder were added to the MS medium to adjust the pH value of the medium to 5.8; the culture conditions were 25±2°C, light intensity 2000-3000lx, light for 14h / d.

[0031] (2) Adventitious bud induction pre-culture

[0032]The stem segments, embryo tips and hypocotyls of Caragana japonica w...

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Abstract

The invention discloses an efficient in-vitro regeneration system for caragana korshinskii. According to the method, embryonic tips of the caragana korshinskii serve as starting materials, a large number of adventitious buds are generated through induction of appropriate hormones, the adventitious buds are elongated under the appropriate hormones, effective in-vitro regeneration of the caragana korshinskii is finally achieved, a large number of regenerated plants can be obtained without being limited by seasons, and a foundation is laid for genetic transformation of the caragana korshinskii. The established efficient embryonic tipin-vitro regeneration method for the caragana korshinskii promotes large-scale propagation of caragana korshinskii seedlings, provides technical guarantee for cultivation of caragana korshinskii plants with excellent characters, research on stress resistance molecular mechanisms of the plants and the like, and fills the blank in the field of caragana korshinskii breeding.

Description

technical field [0001] The invention relates to the fields of plant tissue culture and biotechnology, in particular to a method for in vitro regeneration of Caragana caragana. Background technique [0002] Caragana korshinskii (Caragana korshinskii) belongs to the large deciduous shrub of the legume family Caragana genus, also known as Maotiao and white Caragana. It is a perennial shrub that grows in desert and semi-desert areas (Wang Zan, Gao Hongwen, Han Jianguo. DNA extraction of Caragana korshinskii And the establishment of AFLP reaction system [J]. Cao Di Xue Xue, 2005,13 (2): 126-129). With the rapid advancement of desertification control work, Caragana, as the dominant plant in desert areas, has been highly valued. Caragana has a strong ability to adapt to the sandy environment. It is windproof and sand-fixing, maintains water and soil, improves soil trace element content, has high feeding and medicinal value, and shows strong stress resistance and adaptability. Exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 刘槟朱木兰王瑞刚齐力旺李国婧郑珂媛
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY