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Culture medium for strain tissue isolation and preparation method thereof

A tissue separation and culture medium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and separation of microorganisms, can solve the problems of affecting the full bottle time of strains, poor mycelium growth state, and poor mycelium germination state. , to achieve the effect of improving the tissue separation effect, good mycelial growth state, and good germination state

Pending Publication Date: 2021-08-03
韶关市星河生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the cultivation technology of edible fungus, when the existing medium is cultivating strains, the germination state of the mycelia is poor when the tissue is separated in the medium, the growth of the mycelium is slow, and the growth is uneven. Poor silk condition
The specific reason is that the existing culture medium formula is not suitable for promoting mycelia germination in tissue separation, resulting in slow growth of mycelium and poor growth state of mycelia in test tubes or plates.
Only the mycelia with poor growth status are used in the strain fruiting, which will directly affect the quality of the strain and the full bottle time of the strain

Method used

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  • Culture medium for strain tissue isolation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0025] In this example, a culture medium for strain tissue separation is provided, which includes 15 parts of glucose, 1 part of yeast extract, 1 part of dipotassium hydrogen phosphate, 0.5 parts of magnesium sulfate, 20 parts of agar and 800 parts of water according to the mass ratio , also includes biotin extract and nitrogen source in 16 grain grains.

[0026] Wherein, corn kernels are used as grain grains.

Embodiment 2

[0028] In this example, a culture medium for strain tissue separation is provided, which includes 20 parts of glucose, 1 part of yeast extract, 1 part of dipotassium hydrogen phosphate, 0.5 parts of magnesium sulfate, 20 parts of agar and 1000 parts of water according to the mass ratio , also includes biotin extract and nitrogen source in 18 grain grains.

[0029] Wherein, wheat grains are used as grain grains.

Embodiment 3

[0031] In this example, a culture medium for strain tissue separation is provided, which includes 25 parts of glucose, 1 part of yeast extract, 2 parts of dipotassium hydrogen phosphate, 2 parts of magnesium sulfate, 30 parts of agar and 900 parts of water according to the mass ratio , also includes biotin extract and nitrogen source in 20 grain grains.

[0032] Among them, bran is used as grain grain.

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Abstract

The invention relates to the technical field of edible mushroom planting, and discloses a culture medium for strain tissue isolation, which comprises 15-30 parts of glucose, 1-3 parts of yeast extract, 1-3 parts of dipotassium phosphate, 0.5-3 parts of magnesium sulfate, 20-30 parts of agar, 800-1000 parts of water, 16-28 parts of biotin extract in grains and a nitrogen source. The invention also provides a preparation method of the culture medium for strain tissue isolation, which comprises the following steps: weighing 16-28 parts of grain grains, adding 800-1000 parts of water, boiling for 8-12 minutes with soft fire, filtering and taking filtrate; adding 15-30 parts of glucose, 1-3 parts of yeast extract, 1-3 parts of dipotassium phosphate, 0.5-3 parts of magnesium sulfate and 20-30 parts of agar into the filtrate, uniformly stirring, boiling, cooling, and sub-packaging into test tubes or plates for later use. According to the culture medium for strain tissue isolation, the germination state of hyphae is effectively improved, and the production efficiency of strains is improved.

Description

technical field [0001] The invention relates to the technical field of edible fungus planting, in particular to a culture medium for separating strains and tissues and a preparation method thereof. Background technique [0002] In the cultivation technology of edible fungus, when the existing medium is cultivating strains, the germination state of the mycelia is poor when the tissue is separated in the medium, the growth of the mycelium is slow, and the growth is uneven. The wire is in poor condition. The specific reason is that the existing culture medium formula is not suitable for promoting mycelia germination in tissue separation, resulting in slow growth of mycelium and poor growth state of mycelia in test tubes or plates. Only the hyphae with poor growth state are used in the strain fruiting, which will directly affect the quality of the strain and the full bottle time of the strain. Contents of the invention [0003] The purpose of the present invention is to prov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/14C12N1/02
Inventor 林文英许喜佳黄清华程万杰陈长青梁萍芳
Owner 韶关市星河生物科技有限公司
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