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Application of citronellol in the preparation of preparations for promoting the expression of Pseudomonas aeruginosa virulence gene toxa

A technology of Pseudomonas aeruginosa and citronellol, applied in the field of microorganisms, can solve the problems of easy conversion to chronic infection and deterioration of host organ infection

Active Publication Date: 2022-06-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acute infection of Pseudomonas aeruginosa can be treated with antibiotics, but when Pseudomonas aeruginosa is successfully colonized in the host, it is easy to convert to chronic infection, showing a permanent biofilm state, causing the deterioration of host organ infection, traditional antibacterial therapy It is difficult to completely eradicate it

Method used

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  • Application of citronellol in the preparation of preparations for promoting the expression of Pseudomonas aeruginosa virulence gene toxa
  • Application of citronellol in the preparation of preparations for promoting the expression of Pseudomonas aeruginosa virulence gene toxa
  • Application of citronellol in the preparation of preparations for promoting the expression of Pseudomonas aeruginosa virulence gene toxa

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Experimental program
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Embodiment 1

[0018] Preparation of PAO1 bacteria [Pseudomonas aeruginosa (Pseudomonas aeruginosa) PAO1] suspension: The culture medium of Pseudomonas aeruginosa PAO1 in exponential growth phase was sampled, centrifuged, washed once with PBS buffer, resuspended in PBS, and Dilute the bacterial concentration to 10 8 CFU / mL to obtain PAO1 bacterial suspension.

[0019] 1. The effect of citronellol on the growth of Pseudomonas aeruginosa PAO1

[0020] Add LB medium and citronellol (β-citronellol) to the test tubes respectively, and insert the PAO1 bacterial suspension in the exponential growth phase, the total volume is 10 mL, so that the bacterial concentration of PAO1 is 10. 6 The concentrations of CFU / mL, citronellol were 0 (control), 0.313 μL / mL, 0.625 μL / mL, 1.25 μL / mL and 2.5 μL / mL, respectively. Samples from several experimental groups were added to the honeycomb culture plate dedicated to the automatic growth curve analyzer (Bioscreen C), and 350 μL of culture medium was added to eac...

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Abstract

The invention discloses the application of citronellol in the preparation of a preparation for promoting the expression of the virulence gene toxA of Pseudomonas aeruginosa. The present invention finds that citronellol slightly inhibits the growth of Pseudomonas aeruginosa PAO1 strain, but can promote the transcription of Pseudomonas aeruginosa toxA, thereby increasing the yield of the coding product of toxA - exotoxin A. Therefore, citronellol can be used in the preparation of preparations for promoting the expression of Pseudomonas aeruginosa virulence gene toxA.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to the application of citronellol in the preparation of a preparation for promoting the expression of the virulence gene toxA of Pseudomonas aeruginosa. Background technique: [0002] Pseudomonas aeruginosa, also known as Pseudomonas aeruginosa, often causes cystic fibrosis (CF), meningitis, abscesses, soft tissue infections, urinary tract infections, corneal infections, and ventilator-associated pneumonia and catheter-related Infection, highly susceptible to convalescent patients with weakened immunity, leading to postoperative wound infection. Acute infection of Pseudomonas aeruginosa can be treated with antibiotics, but when Pseudomonas aeruginosa successfully colonizes the host, it is easy to convert to chronic infection, showing a permanent biofilm state, causing the deterioration of host organ infection, traditional antibacterial therapy It is difficult to eradicate...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02C07K14/21C12R1/385
CPCC12N1/20C12P21/02C07K14/21Y02A50/30C12R2001/385
Inventor 李文茹谢小保曾桃花张志清刘静霞施庆珊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY