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A method for establishing a seashore paspalum tissue culture regeneration system

A seashore paspalum and tissue culture technology, applied in the biological field, can solve the problems of providing enough explants for explants, hindering the experimental process, etc., so as to shorten the time of differentiation and culture, improve the induction rate, and shorten the time of induction and culture. Effect

Active Publication Date: 2021-12-17
SICHUAN AGRI UNIV
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Problems solved by technology

Moreover, there is currently only one commercial seed direct-seeding seaside paspalum variety in the world
Therefore, the selection of explants in the existing tissue culture method is limited by the season and material variety and cannot provide enough explants for experiments throughout the year, which greatly hinders the experiment process

Method used

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  • A method for establishing a seashore paspalum tissue culture regeneration system
  • A method for establishing a seashore paspalum tissue culture regeneration system
  • A method for establishing a seashore paspalum tissue culture regeneration system

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Embodiment

[0036] Wash and sterilize multiple seashore paspalum stolon sections and inoculate them into the above induction medium SI, and then culture them in the dark at 25°C for 12 days, subtract the new shoots from seashore paspalum stolons with joints and replace them with new ones. The induction medium SI was cultured continuously for 12 days and obtained as follows: figure 1 The picture of seashore paspalum callus induction is shown. Observe the quality of callus and count the number of callus to calculate the callus induction rate. The callus induction rate is as high as 90%, and the callus growth rate is fast and the quality is high. Preferably, pale yellow, dense, larger (>1 cm in diameter) particles.

[0037] The main function of subculture is to expand the callus, which is more conducive to the cultivation of more regenerated plants. The callus obtained through induction medium SI culture was transferred to the above-mentioned subculture medium SS, and then cultured in the d...

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Abstract

The invention discloses a method for establishing a seashore paspalum tissue culture regeneration system which can overcome the seasonal limitation of explant supply and greatly shorten the experimental period. The present invention uses seashore paspalum stolons as explants, cleans and sterilizes seashore paspalum stolons, cuts them into segmented segments and inoculates them into induction medium SI for 20-30 days to obtain callus, and then transfers the callus into Cultured in the subgeneration medium SS for 5-10 days, then transferred the callus to the differentiation medium SR and cultured for 15-20 days, when the seedlings grew to 2-3 cm, they could be transplanted, and established by the above method The efficient tissue culture regeneration system of seashore paspalum lays the foundation for the establishment of an efficient genetic transformation system of seashore paspalum, and provides a technical reference for the breeding of fine seashore paspalum varieties. At the same time, it overcomes the time limit of explant supply. It takes 40‑60d, which greatly shortens the experiment time. It is suitable for promotion and application in the field of biotechnology.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a seashore paspalum tissue culture regeneration system. Background technique [0002] Seashore paspalum (Paspalum vaginatum Sw.), also known as Hawaiian grass, is a perennial herbaceous plant belonging to the genus Paspalum of the Gramineae family (Paniceae). It is a perennial warm-season turfgrass of the Poaceae family with stolons and rhizomes, which grows on the seashore and prefers warmth. It has sexual reproduction and cross-pollination. It has a certain degree of self-incompatibility and is mainly diploid ( 2n=20). Its color is dark green, requires less fertilizer, has fewer pests and diseases, is resistant to low pruning, drought resistance, weak light and poor soil. It is widely used in sports fields and leisure green space systems from warm temperate to subtropical and tropical regions of the world. [0003] As a halophyte, seaside pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 赵俊茗马啸熊艳丽熊毅刘伟刘琳余青青
Owner SICHUAN AGRI UNIV
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