Application of Jak3 inhibitor to preparation of medicine for treating acute respiratory distress syndrome
An acute respiratory distress and inhibitor technology, applied in the field of medicine, to achieve the effect of inhibiting the release of inflammatory factors, inhibiting neutrophil infiltration, and improving lung injury
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[0014] 24 male C57BL / 6 mice were randomly divided into 4 groups, namely solvent control group, ARDS model group (LPS injection), PF-06651600 group and PF-06651600 drug intervention ARDS group, 6 mice in each group, PF-06651600 ( 30mg / kg) was administered by intragastric administration, LPS (20mg / kg) was administered by intraperitoneal injection, and the solvent control group was given the same amount of normal saline. The PF-06651600 group and the PF-06651600 drug-intervention ARDS group were intragastrically administered PF-06651600 15 minutes before LPS injection. Eighteen hours later, the serum of the mice in each group was collected by orbital blood collection, and then the mice were sacrificed, and the lungs were dissected and used 10% formalin was fixed, lung injury and neutrophil infiltration were detected by hematoxylin & eosin staining and scoring, and the contents of IL-6, TNF-α and other inflammatory factors in serum were detected by ELISA.
[0015] The pathological...
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