Application of polygonatum odoratum polysaccharide in preparation of medicine for treating lung injury
A polysaccharide from Polygonatum polysaccharide and lung injury technology, applied in the application field of medicine, can solve the problems of unsatisfactory prognosis and lack of treatment methods for patients
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Embodiment 1
[0036] The embodiment of the present invention provides a preparation method of Polysaccharide Polysaccharide:
[0037] Weigh 2kg of Yuzhu decoction pieces, crush them with a shredder, add distilled water at a liquid-material ratio of 10:1, stir evenly with a glass rod, and soak for 1 hour to fully mix and soak the medicine and water, heat to a slight boil, and start timing. 2h. After the medicinal liquid was cooled to room temperature, filter the medicinal residues with 9 layers of gauze. The filtered medicinal residues were extracted for the second time at a liquid-to-material ratio of 8:1. The extraction time was 2 hours. The two filtrates were combined and stored at 4°C. Use 95% ethanol to adjust the final concentration of ethanol to 80%, and let stand at 4°C for alcohol precipitation overnight. The ethanol in the precipitate was completely spun out with a rotary evaporator, and 254 g of Polysaccharide Polysaccharide was obtained after freeze-drying, which was a pale yell...
Embodiment 2
[0039] The embodiment of the present invention provides a preparation method of Polysaccharide Polysaccharide:
[0040] Weigh 2kg of Yuzhu decoction pieces, crush them with a crusher, add distilled water at a liquid-material ratio of 9:1, stir evenly with a glass rod, and soak for 1 hour, so that the medicine and water are fully mixed and soaked, heated to a slight boil, and the timing is started. 2h. After the medicinal liquid was cooled to room temperature, the medicinal residues were filtered with 9 layers of gauze, and the filtered medicinal residues were extracted for the second time at a liquid-to-material ratio of 7:1. The extraction time was 2 hours. The two filtrates were combined and stored at 4°C. Use 95% ethanol to adjust the final concentration of ethanol to 75%, and let it stand at 4°C for alcohol precipitation overnight. The ethanol in the precipitate was completely spun out with a rotary evaporator, and 246 g of Polysaccharide Polysaccharide was obtained after...
Embodiment 3
[0042] The embodiment of the present invention provides a preparation method of Polysaccharide Polysaccharide:
[0043]Weigh 2kg of Yuzhu decoction pieces, crush them with a crusher, add distilled water at a liquid-to-material ratio of 11:1, stir evenly with a glass rod, and soak them for 1 hour, so that the medicine and water are fully mixed and soaked, heated to a slight boil, and the timing is started. 2h. After the medicinal liquid was cooled to room temperature, the medicinal residue was filtered with 9 layers of gauze, and the filtered medicinal residue was extracted for the second time at a liquid-material ratio of 9:1. The extraction time was 2 hours. The two filtrates were combined and stored at 4 °C. Use 95% ethanol to adjust the final concentration of ethanol to 85%, and let stand at 4°C for alcohol precipitation overnight. The ethanol in the precipitate was completely spun out with a rotary evaporator, and 249 g of Polysaccharide Polysaccharide was obtained after ...
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