Application of STCH to preparation of functional product for resisting flavivirus infection

A technology of virus infection and flavivirus, applied in the field of biomedicine, can solve problems such as infection and achieve good application prospects

Active Publication Date: 2021-08-06
大汉生物科技(广东)有限公司
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no effective drug for preventing and treating flavivirus infection. In order to solve the harm caused by flavivirus infection and transmission, it is urgent to develop a Novel effective agent or drug against flavivirus infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of STCH to preparation of functional product for resisting flavivirus infection
  • Application of STCH to preparation of functional product for resisting flavivirus infection
  • Application of STCH to preparation of functional product for resisting flavivirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Determination of STCH gene transcription level after flavivirus infection of A549 cells

[0030] 1. Collection of test samples:

[0031] Total RNA samples of A549 cells infected with ZIKV, DENV, JEV and YFV were collected.

[0032] 2. The detection method specifically includes the following steps:

[0033] (1) A549 cells according to 2×10 5 cells / ml in 12-well plates and cultured overnight.

[0034] (2) Discard the culture medium the next day and wash twice with 1×PBS. Take DENV infection as an example: Infect cells at MOI=1, absorb for 1 hour, and shake well every 15 minutes. After 1h, discard the adsorption solution, wash twice with FBS-free DMEM, add 1ml maintenance solution, and incubate for 48h.

[0035] (3) Total cellular RNA was collected at 0h, 24h and 48h, and Quantitative Real-time PCR (Q-RT-PCR) was used to detect the transcription level of STCH gene.

[0036] 3. Experimental results:

[0037] The result is as figure 1 The results showed tha...

Embodiment 2

[0038] Example 2 Determination experiment of flavivirus gene transcription level after overexpressing STCH in A549 cells

[0039] 1. Collection of test samples:

[0040] Total RNA samples of A549 cells infected with DENV, JEV, YFV and ZIKV after overexpression of STCH were collected.

[0041] 2. The detection method specifically includes the following steps:

[0042] (1) A549 cells according to 2×10 5 cells / ml in 12-well plates and cultured overnight.

[0043] (2) Discard the culture medium the next day and wash twice with 1×PBS. Taking DENV infection as an example: an HA-tagged STCH overexpression plasmid (HA-STCH, lug / ul) was constructed, transfected into A549 cells, and then the cells were infected with the virus at MOI=1, and cultured for 24 hours.

[0044] (3) Total cellular RNA was collected, and the transcription level of the virus was detected by Q-RT-PCR.

[0045] 3. Experimental results:

[0046] The result is as figure 2 It was shown that the gene transcriptio...

Embodiment 3

[0047] Example 3 Determination of Flavivirus Protein Transcription and Gene Translation Levels After Overexpression of STCH in A549 Cells

[0048] 1. Collection of test samples:

[0049] The total RNA samples and total protein samples of A549 cells infected with ZIKV and DENV after overexpression of STCH were collected.

[0050] 2. The detection method specifically includes the following steps:

[0051] (1) A549 cells according to 2×10 5 cells / ml in 12-well plates and cultured overnight.

[0052] (2) Discard the culture medium the next day and wash twice with 1×PBS. Taking DENV infection as an example: an HA-tagged STCH overexpression plasmid (HA-STCH, lug / ul) was constructed, transfected into A549 cells, and then the cells were infected with the virus at MOI=1, and cultured for 24 hours.

[0053] (3) Total cellular RNA and total protein were collected, and the transcription and translation levels of viral proteins were detected by Q-RT-PCR and Western Blot.

[0054] 3. E...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of biological medicine, and discloses an application of a stress chaperone molecule (STCH) to preparation of a functional product for resisting flaviviruses. By flavivirus inhibition experiments, the invention proves that an STCH overexpression vector has a remarkable inhibition effect on the flaviviruses, which shows that the STCH overexpression vector is expected to prevent and treat diseases caused by a flavivirus infection when being applied to preparation of a drug for resisting the flavivirus infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically relates to the application of STCH in the preparation of functional products resistant to flavivirus infection. Background technique [0002] Flavivirus (Flavivirus) belongs to the Flavivirus genus of the family Flaviviridae. It is a large group of enveloped single positive-strand RNA viruses with a diameter of 40-70 nm and a icosahedral stereosymmetry. This type of virus causes infection through the transmission of blood-sucking arthropods (mosquitoes, ticks, sandflies, etc.), and was classified as arboviruses in the past. At present, those that have a greater impact on humans include Dengue Virus (DENV), Zika Virus (ZIKV), Japanese Encephalitis Virus (JEV), West Nile Virus (WNV) ) and yellow fever virus (Yellow Fever Virus, YFV) and so on. [0003] Diseases caused by dengue virus (DENV) infection include: general fever, mild dengue fever (dengueFever, DF), severe den...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K38/17A61K48/00A61P31/14
CPCA61K38/1709A61K48/005A61P31/14Y02A50/30
Inventor 黄曦王巧花吴永坚
Owner 大汉生物科技(广东)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap