Impregnation rotary spinning equipment for preparing gelatin/cellulose 3D scaffold for cell culture meat production

Abstract

The invention relates to impregnation rotary spinning equipment for preparing a 3D scaffold for cell culture meat production. A gelatin / cellulose 3D scaffold manufactured by the equipment can be used for culturing skeletal muscle cells, support is provided for manufacturing cell culture meat, and the equipment belongs to the field of future food science and technology. The method comprises the following steps: driving a sample storage device to rotate at a certain speed (100-200r / min), then adding a gelatin / cellulose solution mixed according to a certain proportion into the sample storage device, throwing the mixed solution into a shaping solution (ethanol solution) in a collecting cylinder in a filamentous form through a sample outlet hole in the side of the bottom of the sample storage device, fixing the gelatin / cellulose mixture into filamentous fibers in the shaping solution, and further stacking the filamentous fibers to form the loose and porous gelatin / cellulose 3D scaffold. The gelatin / cellulose 3D scaffold has good cell compatibility, and skeletal muscle cell culture can be carried out on the 3D scaffold. The invention provides a good device and method for preparing the cell culture meat 3D scaffold.

Description

technical field [0001] The invention relates to the field of future food science and technology, in particular to an impregnated rotary spinning device for producing 3D scaffolds for skeletal muscle cell culture and the preparation of gelatin / cellulose 3D scaffolds. Background technique [0002] According to the data of the United Nations, it is estimated that by 2030, the global urban population will increase to 60%, and the global meat consumption will exceed 300 million tons. It is estimated that by 2050, the global population will soar to 9.6 billion, and more livestock-based products will be consumed, and the total meat consumption is expected to exceed 400 million tons. Meat production often requires a more intensive and environmentally harmful method of production, and farming consumes about 30 percent of the world's food each year. With a projected 61% increase in food production by 2050, the environmental impact of the food system could increase by 50% to 92% and r...

AI Technical Summary

Problems solved by technology

In the past, the reconstruction of fiber gelatin often used methods such as electrospinning or phase separation, but electrospinning has high requirements for the conductivity and viscosity of the solution itself, and some toxic organic solvents need to be added to meet the requirements of electrospinning. Spinning requirements, in this case, the construction of fibrous gelatin using electrospinning has certain cytotoxicity and is not suitable for the preparation of 3D scaffolds in the food field

In addition, the low productivity of electrospinning or phase separation also limits their wide application in food production.

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