Application of dimethyl dicarbonate in preparation of postharvest citrus preservative

A technology of dimethyl dicarbonate and a preservative is applied in the application field of preparing the main postharvest disease preservatives and preservatives for citrus, can solve the problems of inhibitory effect and no relevant reports on storage and preservation, and achieves inhibition of citrus Green mold and sour rot, the effect of improving the ability to keep fresh and enhancing the defense ability

Active Publication Date: 2021-08-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no related reports on the inhibitory effect of dimethyl dicarbonate on postharvest Penicillium digitatum and Acid rot fungus and storage and preservation of citrus

Method used

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  • Application of dimethyl dicarbonate in preparation of postharvest citrus preservative
  • Application of dimethyl dicarbonate in preparation of postharvest citrus preservative
  • Application of dimethyl dicarbonate in preparation of postharvest citrus preservative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Inhibition experiments of different concentrations of dimethyl dicarbonate on P.digitatum and G.citri-candidum:

[0043] In the present embodiment, in the 96-well plate experiment, different concentrations were used to measure the mycelial growth inhibition of Penicillium citrus digitatum and Sour rot fungus. 4 CFU / mL, and add dimethyl dicarbonate solution (diluted with absolute ethanol 1:4 to 312.5g / L) to make the final concentration 0.032g / L, 0.062g / L, 0.125g / L, 0.25 g / L, 0.5g / L, 1g / L, with absolute ethanol as the control. Add 200μL to a 96-well plate and repeat three times. Use 5% PDB to prepare Sour rot fungus spore suspension 1×10 5 CFU / mL, and add dimethyl dicarbonate solution (diluted with absolute ethanol 1:4 to 312.5g / L) to make the final concentration 0.062g / L, 0.125g / L, 0.25g / L, 0.375 g / L, 0.5g / L, 0.625g / L, with absolute ethanol as the control. Add 200μL to a 96-well plate and repeat three times.

[0044] 2. Place the 96-well plate of Penicillium digitat...

Embodiment 2

[0049] The application of low-concentration dimethyl dicarbonate in the preparation and control of postharvest citrus green mold is as follows:

[0050] 1. Select 30 ripe sweet orange fruits with the same size and no mechanical damage, soak in 2% sodium hypochlorite solution for 2 minutes, rinse twice with water, and air-dry at room temperature;

[0051] 2. Mix dimethyl dicarbonate and absolute ethanol at a volume ratio of 1:4 to prepare dimethyl dicarbonate mother liquor (concentration: 312.5g / L), take dimethyl dicarbonate mother liquor in 2L In water, the final concentrations were 1.675g / L and 6.7g / L respectively;

[0052] 3. Divide 30 fruits into 3 groups, with 10 fruits in each group. The fruits of the first group were immersed in 0.25% absolute ethanol for 1 min, and the fruits of the second group were immersed in 1.675 g / L dimethyl dicarbonate for 1 min; The third group was immersed in 6.7g / L dimethyl dicarbonate for 1min;

[0053] 4. Configure 1×10 with sterile water ...

Embodiment 3

[0063] The application of dimethyl dicarbonate in the preparation and prevention of postharvest citrus sour rot, its application method is:

[0064] 1. Select 27 ripe sweet orange fruits with the same size and no mechanical damage, soak them in 2% sodium hypochlorite solution for 2 minutes, rinse them twice with water, and air-dry them at room temperature;

[0065] 2. Mix DMDC and absolute ethanol at a volume ratio of 1:4 to prepare dimethyl dicarbonate mother liquor (concentration is 312.5g / L), take dimethyl dicarbonate mother liquor in 2L distilled water, and the final concentrations are respectively 1.34g / L and 6.7g / L;

[0066] 3. Divide 27 fruits into 3 groups, 9 fruits in each group. The first group of fruits was immersed in 0.25% absolute ethanol for 1 min, and the second group was immersed in 1.34 g / L dimethyl dicarbonate for 1 min. The third group was immersed in 6.7g / L dimethyl dicarbonate for 1min, and air-dried in an ultra-clean workbench.

[0067] 4. Configure 5×...

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Abstract

The invention belongs to the field of preservatives for postharvest storage of fruits, and discloses application of dimethyl dicarbonate in preparation of a postharvest citrus preservative. In-vitro, fruit living body and producing area storage experiments prove that the dimethyl dicarbonate can effectively inhibit the occurrence of Penicillium citrinum and sour rot, is suitable for preservation and fresh keeping of citrus fruits, and clarifies the application prospect of the dimethyl dicarbonate in postharvest treatment of citrus. Meanwhile, the dimethyl dicarbonate is a food additive which can be added into the beverage, and the safety of the dimethyl dicarbonate can be determined. The dimethyl dicarbonate is used as a preservative for the first time at home and abroad to be applied to the postharvest treatment of the citrus fruits.

Description

technical field [0001] The invention belongs to the field of postharvest storage preservatives for fruits, and in particular relates to the application of dimethyl dicarbonate in the preparation of antiseptics and preservatives for main postharvest diseases of citrus. Background technique [0002] Citrus is a Rutaceae citrus plant. It is the most widely planted fruit with the highest yield in the world, and it is also one of the main fruits in my country. In the domestic citrus industry, citrus fruits mainly sold fresh account for 95% of the total output ( Deng Xiuxin 2004). Citrus is very easy to rot due to environmental changes during storage and transportation, and the rot rate can be as high as 30%, which reduces economic benefits. (Ma Yaqin et al. 2019) Citrus rot is mainly caused by invasive fungal diseases. More than 20 kinds of postharvest fungal diseases of citrus have been reported, among which the main diseases are citrus green mold, sour rot, and penicillium, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
CPCA23B7/154Y02A40/90
Inventor 龙超安刘书琪张德遥杨凡
Owner HUAZHONG AGRI UNIV
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