Thin layer chromatography identification method for golden cypress and golden cypress wine roasted product

A technology of thin-layer chromatography and wine-burning products, which is applied to the analysis of materials, measuring devices, instruments, etc., can solve the high requirements of experimental reagent quality and personnel operation, increase the difficulty of rapid identification of cork cortex and wine cortex, and the experimental steps To achieve the effect of simple operation, clear and stable spot separation, and good repeatability

Pending Publication Date: 2021-08-13
山西省药品审评中心
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research group tried many times to use the thin-layer chromatography method of Phellodendron cortex contained in the "Chinese Pharmacopoeia" (2020 edition) to identify Phellodendron cortex and Phellodendron japonica, and found that there w

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thin layer chromatography identification method for golden cypress and golden cypress wine roasted product
  • Thin layer chromatography identification method for golden cypress and golden cypress wine roasted product
  • Thin layer chromatography identification method for golden cypress and golden cypress wine roasted product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1 material

[0069] 1.1 Main instruments and materials

[0070] AB135-S Electronic Balance (METTLER); SB25-12DTD Ultrasonic Cleaner (Ningbo Xinzhi); CBIO21 Multifunctional Black Box UV Analyzer (Beijing Saibaio); CY Drum Roasting Machine (Nanjing Tengyang) ; Double-slot chromatography cylinder (base solid glass). Silica gel G plate (batch number: 020190411, Yantai Jiangyou); silica gel GF254 plate (batch number: 20061021, Qingdao Haiyang); micro capillary (1 μL, batch number: 889892; 3 μL, batch number: 895254, Germany, HIRSCHMANN).

[0071] 1.2 medicinal materials

[0072] Phellodendron chinense decoction pieces (Phellodendron si) used (fifteen batches of Phellodendron chinense and three batches of Guan Phellodendron) were purchased from Anguo, Hebei, and were identified as dry bark of Phellodendron chinense Schneid. and the dry bark of Phellodendron amurense Rupr. The cortex decoction pieces obtained are processed to obtain the decoction pieces of cortex cortex. ...

Embodiment 2

[0125] Embodiment 2: Thin-layer chromatography identification specificity investigation

[0126] To investigate the specificity of this method, the results are shown in Figure 7 (Test solution: 1. Cortex Phellodendron 1; 2. Cortex Phellodendron 1; 3. Cortex Phellodendron reference medicinal material; 4. Phellodendronine hydrochloride solution; 5. Berberine hydrochloride solution; sample volume: 3 μL). The test products Cortex Phellodendron 1 and Cortex Cortex 1 had the same fluorescent spots at the same positions as the berberine hydrochloride solution and the Cortex Cortex reference medicinal material, but Cortex Cortex Hydrochloride had no fluorescent spots and no other interference spots. It shows that the specificity of the method is good.

[0127] Adopt this method to carry out spectrum identification investigation to Phellodendron Phellodendri (Sichuan Phellodendron) and Phellodendron officinale, see Figure 13 (need testing solution: 1 Cortex Phellodendron 1; 2. Cort...

Embodiment 3

[0128] Embodiment 3: the investigation of durability

[0129] (1) Investigation of the influence of silica gel plate on the identification of thin layer chromatography

[0130] To investigate the durability of high-efficiency thin-layer boards and ordinary thin-layer boards produced by three different manufacturers, see Figure 8 (need testing solution: 1. Phellodendron 1; 2. Phellodendron 1; 3. Phellodendron 7; 4. Phellodendron 13; 5. Phellodendron reference medicine solution; 6. Berberine hydrochloride solution; Silica gel G plate: Ⅰ, Yantai City Jiangyou high-efficiency plate; II, Qingdao ocean ordinary plate; III, Qingdao ocean high-efficiency plate), it is obviously found that the silica gel particles of the high-efficiency plate are uniform and small in size, and the spraying is uniform, so the number of separation plates is high, so berberine hydrochloride The fluorescent spots are relatively concentrated, and the boundaries are clear, which is more conducive to TLC id...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a thin layer chromatography identification method for golden cypress and a golden cypress wine roasted product. The thin layer chromatography identification method comprises the following steps: (1) grinding a sample into powder to obtain medicinal material powder, mixing the obtained medicinal material powder with a first solvent, and extracting in an ultrasonic or heating reflux manner to obtain an extracting solution; (2) carrying out solid-liquid separation on the extracting solution obtained in the step (1), and adding a second solvent into the obtained liquid to prepare a test solution; and (3) carrying out sample application on the test solution and the reference solution obtained in the step (2) on a silica gel plate, spreading, taking out, drying, and inspecting under an ultraviolet lamp. According to the method provided by the invention, after the developing is finished in the developing solvent, the visual inspection is directly performed at 365 nm, so that the method is convenient and visual and the problems that in the prior art, a thin-layer plate needs to be placed in a saturated developing solvent in ammonia steam to be developed, a diluted bismuth potassium iodide test solution needs to be sprayed after development, then inspection is conducted, and operation is complex are solved.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a thin-layer chromatography identification method for Cortex Phellodendri and cork cork wine-burning products. Background technique [0002] Phellodendron chinense Schneid. is the dry bark of Rutaceae Phellodendron chinense Schneid. It is commonly known as "Sichuan Phellodendron". Sexual bitterness, cold. Return kidney, urinary bladder channel. Clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores [1-3] . The main production areas are Sichuan, Guizhou, Hubei, and Yunnan, with Sichuan and Guizhou producing the most [4] . Phellodendron: plate-like or shallow-trough-like, varying in length and width, 3-6mm thick. The outer surface is yellow-brown or yellow-brown, flat or with longitudinal grooves, and some visible lenticels and residual gray-brown rough skin. The inner surface is dark yellow or light brown, with fine longitudinal ribs. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/90G01N30/95G01N30/94G01N30/06G01N30/14
CPCG01N30/90G01N30/95G01N30/94G01N30/06G01N30/14
Inventor 阮文辉叶松华王晓燕冯夏珍吴哲薛润苗
Owner 山西省药品审评中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap