Thin layer chromatography identification method for golden cypress and golden cypress wine roasted product

Abstract

The invention discloses a thin layer chromatography identification method for golden cypress and a golden cypress wine roasted product. The thin layer chromatography identification method comprises the following steps: (1) grinding a sample into powder to obtain medicinal material powder, mixing the obtained medicinal material powder with a first solvent, and extracting in an ultrasonic or heating reflux manner to obtain an extracting solution; (2) carrying out solid-liquid separation on the extracting solution obtained in the step (1), and adding a second solvent into the obtained liquid to prepare a test solution; and (3) carrying out sample application on the test solution and the reference solution obtained in the step (2) on a silica gel plate, spreading, taking out, drying, and inspecting under an ultraviolet lamp. According to the method provided by the invention, after the developing is finished in the developing solvent, the visual inspection is directly performed at 365 nm, so that the method is convenient and visual and the problems that in the prior art, a thin-layer plate needs to be placed in a saturated developing solvent in ammonia steam to be developed, a diluted bismuth potassium iodide test solution needs to be sprayed after development, then inspection is conducted, and operation is complex are solved.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a thin-layer chromatography identification method for Cortex Phellodendri and cork cork wine-burning products. Background technique [0002] Phellodendron chinense Schneid. is the dry bark of Rutaceae Phellodendron chinense Schneid. It is commonly known as "Sichuan Phellodendron". Sexual bitterness, cold. Return kidney, urinary bladder channel. Clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores [1-3] . The main production areas are Sichuan, Guizhou, Hubei, and Yunnan, with Sichuan and Guizhou producing the most [4] . Phellodendron: plate-like or shallow-trough-like, varying in length and width, 3-6mm thick. The outer surface is yellow-brown or yellow-brown, flat or with longitudinal grooves, and some visible lenticels and residual gray-brown rough skin. The inner surface is dark yellow or light brown, with fine longitudinal ribs. ...

AI Technical Summary

Problems solved by technology

The research group tried many times to use the thin-layer chromatography method of Phellodendron cortex contained in the "Chinese Pharmacopoeia" (2020 edition) to identify Phellodendron cortex and Phellodendron japonica, and found that there were many experimental steps and poor repeatability, which seriously affected the quality of experimental reagents and personnel. The operation requirements are high, which increases the difficulty of rapid identification of cork and wine cork

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