Method for extracting unsaturated fatty acid from bischofia polycarpa fruits

A technology of unsaturated fatty acids and Chongyang wood, which is applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of low oil yield, unsuitable extraction of Chongyang wood unsaturated fatty acids, and complicated supercritical process, so as to improve the extraction efficiency, shortening the leaching time, shortening the effect of extraction time

Inactive Publication Date: 2013-05-22
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low oil yield of the traditional pressing method and the complicated process of the supercritical method, they are not suitable for extracting unsaturated fatty acids from Chongya

Method used

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  • Method for extracting unsaturated fatty acid from bischofia polycarpa fruits
  • Method for extracting unsaturated fatty acid from bischofia polycarpa fruits
  • Method for extracting unsaturated fatty acid from bischofia polycarpa fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the method for extracting unsaturated fatty acid from the fruit of Chrysanthemum chrysalis, carries out the following steps successively:

[0017] 1) Dry the collected fruit of Chongyang tree at low temperature (50°C) to constant weight (when weighed on a ten-thousandth balance, the weight remains unchanged after 24 hours), and then crushed until it can pass through a 60-mesh sieve. Obtain the crushed Chongyang tree fruit;

[0018] 2) According to the solid-liquid ratio of 1g: 7ml, weigh 10g of crushed fruit of Chongyang tree and add 70ml of n-hexane as a solvent; ultrasonically extract for 1h at an ultrasonic power of 300w and an extraction temperature of 25°C (ultrasonic is limited by Kunshan Instruments) The CNC ultrasonic cleaner produced by the company, the model is KQ-500DE, the same below); the extract and residue are obtained separately;

[0019] 3) Wash the residue with n-hexane for 3 times, the amount of n-hexane used for each washing is 4 ml; v...

Embodiment 2

[0022] Embodiment 2, fatty acid methyl esterification in the fruit

[0023] Accurately measure 200 μl of the fruit oil obtained in Example 1 into a 10ml volumetric flask, then add 1ml of n-hexane and diethyl ether (V:V=2:1) ​​mixed solution, shake gently for 2min, and make the oil (Chongyang tree fruit oil) completely dissolved. Then add 1ml of 0.5mol / L sodium hydroxide-methanol solution, let stand at room temperature (20~25°C) for 5-10min, add distilled water to make up to 10ml, oscillate and shake well, and after mixing, sonicate for 2min under the ultrasonic instrument (The power is 300W, the temperature is 25°C), and finally the mixed solution is transferred to a 10ml centrifuge tube and centrifuged at 4000rpm for 2min. A total of 500 μl of methylated fatty acid was obtained after dehydration of the upper layer solution. The methylated fatty acid was subjected to GC-MS analysis.

Embodiment 3

[0024] Example 3, using GC-MS (instrument is: Thermo Scientific gas chromatography, gas phase model is TRACE GC ULTRA; mass spectrometer model is ISQ; automatic sampler model is AS TriPLUS) to analyze the composition of the fruit fatty acids of Chongyang tree.

[0025] Gas chromatography conditions: chromatographic column (model: TR-5MS: 30m×0.25mm×0.25μm), solvent is n-hexane, carrier gas is high-purity helium (99.999%), heating program: initial temperature of 190°C for 5 minutes, heating The speed was 3°C / min, the stop temperature was 250°C and kept for 5 minutes, the temperature of the injection port was 220°C, the injection volume was 1 μL, and the split injection was performed with a split ratio of 100:1.

[0026] Mass spectrometry conditions: EI ionization source. The transfer line temperature was 250°C, the ion source temperature was 200°C, and the scan range was 40-600 amu.

[0027] The composition of embodiment 2 gained is carried out GC-MS analysis, obtains the tota...

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Abstract

The invention discloses a method for extracting unsaturated fatty acid from bischofia polycarpa fruits. The method comprises the following steps: 1) crushing the bischofia polycarpa fruits which are dried to reach constant weight, thus obtaining the crushed bischofia polycarpa fruits; 2) adding normal hexane serving as a solvent to the crushed bischofia polycarpa fruits based on the solid-to-liquid ratio of 1g: 5-8ml, and ultrasonically extracting for 0.8 to 1.2 hours at ultrasonic power of 200 to 500w and extraction temperature of 25 DEG C, and then respectively extracting the liquid and the residue; 3) cleaning the residue through the normal hexane so as to obtain the washing solution; and 4) combining the extracting solution with the washing solution, and carrying out rotary evaporation to recover the normal hexane, so as to obtain the bischofia polycarpa fruits containing the unsaturated fatty acid. The method for extracting the unsaturated fatty acid from the bischofia polycarpa fruits has the characteristics of being simple in process, high in yield and free from pollution in the process.

Description

technical field [0001] The invention belongs to the technical field of plants, and in particular relates to a preparation method for extracting unsaturated fatty acids from woody Chinese herbal medicine Chongyang wood. Background technique [0002] Plant fatty acids have a variety of physiological activities, especially unsaturated fatty acids containing multiple double bonds, which have various functions such as lowering blood fat, stabilizing cell membrane function, anti-cardiovascular disease, promoting growth and development, and health care. Nutrients. α-linolenic acid and linoleic acid are two important polyunsaturated fatty acids, which can prevent and regulate many human diseases. α-linolenic acid can directly participate in catabolism in the human body, and its metabolites include polyunsaturated fatty acids such as DHA and EPA. DHA and EPA are important components of tissue membranes such as the brain and retina, and they have important functions for the nervous ...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B1/04
Inventor 卢泳全王冬梅童再康
Owner ZHEJIANG FORESTRY UNIVERSITY
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