Unlock instant, AI-driven research and patent intelligence for your innovation.

Selenium binding protein 1 detection from body fluids for diagnosis of peracute tissue damage

A tissue injury and acute technique, applied in disease diagnosis, microbial determination/inspection, biological testing, etc., can solve problems such as incomplete specificity of cardiomyocytes

Pending Publication Date: 2021-08-13
贝里索尔股份有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another limitation of the assay according to the prior art is that troponin T and / or troponin I are released by skeletal muscle and cardiomyocytes, so they are simultaneously insensitive relative to other tissues and insensitive to cardiomyocytes. fully specific
Therefore, very acute tissue damage occurring in other organs or tissues cannot be detected by these tests

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Selenium binding protein 1 detection from body fluids for diagnosis of peracute tissue damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] Example 1: SELENBP1 can be detected in blood samples from patients with acute coronary syndrome

[0125] Circulating SELENBP1 was determined in serum samples from patients suspected of having acute coronary syndrome (ACS). Diagnosis was confirmed in parallel by traditional methods. Blood samples were drawn at several time points after the first onset of symptoms suspected to be caused by ACS. Patients had higher mean SELENBP1 concentrations than healthy controls. Concentrations display a dynamic time course with patient-specific features.

Embodiment 2

[0126] Example 2: SELENBP1 in blood of healthy controls

[0127] High quality serum samples from a panel of control adults (n=75) were used to determine normal SELENBP1 levels in the blood of healthy subjects. Only trace concentrations were detectable, mainly below the linear range of the SELENBP1 assay. The values ​​obtained were normally distributed with a mean value of 0.23 nmol / l. The 95th percentile of values ​​for SELENBP1 concentrations in healthy controls was up to 0.32 nmol / l and the 99th percentile was up to 0.39 nmol / l.

Embodiment 3

[0128] Example 3: Expression and purification of recombinant SELENBP1

[0129]Expression of recombinant human SELENBP1 (rhSELENBP1 ) in baculovirus-infected insect cells. Using primers P1 (SEQ: ID NO.2) and P2 (SEQ: ID NO.3) (Invitrogen, Thermo Fischer Scientific, Dreieich, Germany) containing BamHI and HindIII restriction sites, respectively, according to SEQ: ID NO.1 The cDNA sequence encoding rhSELENBP1 (according to UniProtKB database No. Q13228-1) was amplified by PCR. The pFastBac1 plasmid (Thermo Fischer Scientific) was digested with BamHI and HindIII, and the plasmid fragment was removed and replaced by the PCR sequence resulting in the pFastBac2-SelenBP1-His6 plasmid. Transform DH10Bac E. coli cells and identify bacmid positive cells, culture and isolate recombinant bacmid. Sf9 insect cells were transferred with bacmid DNA by Cellfectin (Thermo Fischer Scientific) for obtaining recombinant virus stock for initiating rhSELENBP1 biosynthesis in "High Five" insect susp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention refers to an immunoassay for determining the amount of selenium binding protein 1 (SELENBP1) in the body liquid of an individual suspected of suffering from peracute tissue damage and detecting pathologically elevated levels of SELENBP1, the diagnostic use of this immunoassay and a kit for the performance of a diagnostic assay comprising an antibody against SELENBP1.

Description

technical field [0001] The present invention relates to an immunoassay for determining the amount of selenium-binding protein 1 (SELENBP1) in the body fluid of an individual suspected of having very acute tissue injury and for detecting pathologically elevated levels of SELENBP1, the diagnostic use of the immunoassay and the Kit for performing diagnostic assays for antibodies to SELENBP1. Background technique [0002] Selenium (Se) is an essential trace element for mammals (Schwartz and Foltz (1958) J Biol Chem, 248-251). It is associated with development of type 2 diabetes (Rayman and Stranges (2013) Free Radic Biol Med, 65:1557-1564), cancer (Clark, L.C. et al. (1996) JAMA 276, 1957–1963) and overall mortality (Rayman ( 2012) Lancet, 379:1256-1268). Selenium can be found in various biological forms in the human body. It is primarily incorporated into amino acids such as selenomethionine, selenocysteine, and selenoneine, or into methylated amino acids such as methylselen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/50C12Q1/68G01N33/6887G01N33/74G01N33/6893G01N2333/4703G01N2800/10
Inventor 卢茨·施姆堡E·C·奎恩
Owner 贝里索尔股份有限公司