Micro-fluidic chip and application thereof

Abstract

The invention discloses a micro-fluidic chip and application thereof. The micro-fluidic chip comprises a reaction chip and a filter sheet which is located above the reaction chip and used for filtering and collecting an object to be detected; the reaction chip is provided with a sample outlet, at least one sample inlet and a plurality of functional cavities communicated through flow channels, each sample inlet is correspondingly communicated with a liquid inlet, and the liquid inlet is used for guiding corresponding liquid into the functional cavities or flow channels. On the basis of the micro-fluidic chip technology, an affinity enrichment method and a physical property method are combined, and the two technical means are integrated on the micro-fluidic chip, so that rare cells in a blood sample are separated, captured and enriched, and an enriched cell sample solution with relatively high purity is obtained, and the rare cells in the blood sample are accurately counted through an immunofluorescence staining method; and meanwhile, an immunofluorescence staining detection method is integrated on the micro-fluidic chip, so that human factor interference is avoided, manual errors are reduced, the detection accuracy is improved, the detection speed is increased, the reagent cost and the equipment cost are reduced, and detection engineering can be realized.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a microfluidic chip and its application, especially the application and method of the microfluidic chip in detecting rare cells. Background technique [0002] Rare cells refer to cells with less than 1,000 cells per milliliter of sample. Rare cells in the human circulatory system mainly include circulating tumor cells (Circulating Tumor Cells, CTCs), circulating endothelial cells (Circulating Endothelial Cells, CECs) ), circulating endothelial progenitor cells (cEPCs), stem cells and fetal cells in maternal blood. [0003] The number of rare cells in the circulatory system of different individuals is different, and the number of rare cells in the body is different in a healthy state, in different disease states, and in different stages of the same disease, and rare cells are closely related to diseases, such as , CTCs and circulating tumor microemboli (circulating tum...

AI Technical Summary

Problems solved by technology

[0006] The detection speed of flow cytometry is fast, and multi-channel detection can be performed at the same time, but its detection sensitivity is low, the number of cells is high, and the cell shape cannot be observed; fluorescence in situ hybridization can realize molecular level detection, The clinical application is mature, but when the length of the selected probe is short, it will easily lead to low hybridization efficiency and low accuracy; real-time quantitative PCR can detect RNA in target cells, and the detection sensitivity is high, but it is subject to RNA degradation and contamination. The limitation of characteristics will lead to a higher probability of false positives, which limits its clinical application and promotion. At the same time, the above method requires complex pretreatment of samples, and the consumption of reagents is large, and the cost of reagents and equipment is high.

[0007] Immunofluorescence staining can observe clear cell morphology and has high detection sensitivity. It is the most commonly used detection method at present. However, the observed cell morphology is diverse, and the accuracy of statistical counting is greatly affected by the purity of the sample and the volume of the sample solution. less accurate

[0008] Therefore, the existing detection methods all have the problem of low accuracy when detecting rare cells

Images